Protein profile

PA2664

flavohemoprotein

Genome: NC_002516.2

Gene: fhp PA2664 hmp Structure source: AlphaFold UniProt Q9I0H4
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Amino acids 393
Annotations 13
Features 33
PDB binders 9
Druggability 0.859

Overview

Basic information about this protein and its source genome.

Accession
PA2664
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
fhp PA2664 hmp
Status
annotated
Amino acids
393
Structure source
AlphaFold
EC
1.14.12.17
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0071949 Binding to the oxidized form, FAD, of flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes. GO:0020037 Binding to a heme, a compound composed of iron complexed in a porphyrin (tetrapyrrole) ring. GO:0046872 Binding to a metal ion. GO:0008941 Catalysis of the reaction: 2 NO + 2 O2 + NAD(P)H + H+ = 2 nitrate + NAD(P)+. GO:0019825 Binding to oxygen (O2).

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
25.234
Human E-value
8.82e-08
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.859
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 12 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

12
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0071949 Binding to the oxidized form, FAD, of flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
  • GO:0020037 Binding to a heme, a compound composed of iron complexed in a porphyrin (tetrapyrrole) ring.
  • GO:0046872 Binding to a metal ion.
  • GO:0008941 Catalysis of the reaction: 2 NO + 2 O2 + NAD(P)H + H+ = 2 nitrate + NAD(P)+.
  • GO:0019825 Binding to oxygen (O2).
  • GO:0005344 Binding to oxygen and delivering it to an acceptor molecule or a specific location.
  • GO:0071500 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nitrosative stress stimulus. Nitrosative stress is a state often resulting from exposure to high levels of nitric oxide (NO) or the highly reactive oxidant peroxynitrite, which is produced following interaction of NO with superoxide anions.
  • GO:0046210 The chemical reactions and pathways resulting in the breakdown of nitric oxide, nitrogen monoxide (NO), a colorless gas only slightly soluble in water.
  • GO:0009636 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a toxic stimulus.
  • GO:0016491 Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
  • GO:0051409 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nitrosative stress stimulus. Nitrosative stress is a state often resulting from exposure to high levels of nitric oxide (NO) or the highly reactive oxidant peroxynitrite, which is produced following interaction of NO with superoxide anions.

Sequence Features

Domain/signature hits from InterPro and related databases.

33 records
Show feature table
Start End DB Term Name
1 146 FunFam G3DSA:1.10.490.10:FF:000003 Flavohemoprotein
1 390 PANTHER PTHR43396 FLAVOHEMOPROTEIN
1 142 SUPERFAMILY SSF46458 Globin-like
1 142 InterPro IPR009050 Globin-like superfamily
26 136 ProSiteProfiles PS01033 Globin family profile.
26 136 InterPro IPR000971 Globin
158 253 Pfam PF00970 Oxidoreductase FAD-binding domain
158 253 InterPro IPR008333 Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain
353 361 PRINTS PR00410 Phenol hydroxylase reductase family signature
263 282 PRINTS PR00410 Phenol hydroxylase reductase family signature
186 198 PRINTS PR00410 Phenol hydroxylase reductase family signature
248 257 PRINTS PR00410 Phenol hydroxylase reductase family signature
2 142 CDD cd14780 HmpPa-globin-like
1 145 Gene3D G3DSA:1.10.490.10 Globins
1 145 InterPro IPR012292 Globin/Protoglobin
152 253 Gene3D G3DSA:2.40.30.10 Translation factors
27 132 Pfam PF00042 Globin
27 132 InterPro IPR000971 Globin
153 256 ProSiteProfiles PS51384 Ferredoxin reductase-type FAD binding domain profile.
153 256 InterPro IPR017927 FAD-binding domain, ferredoxin reductase-type
264 367 Pfam PF00175 Oxidoreductase NAD-binding domain
264 367 InterPro IPR001433 Oxidoreductase FAD/NAD(P)-binding
254 391 Gene3D G3DSA:3.40.50.80 -
254 391 InterPro IPR039261 Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
1 391 Hamap MF_01252 Flavohemoprotein [hmp].
1 391 InterPro IPR023950 Flavohemoprotein
255 391 FunFam G3DSA:3.40.50.80:FF:000010 Flavohemoprotein
255 388 SUPERFAMILY SSF52343 Ferredoxin reductase-like, C-terminal NADP-linked domain
255 388 InterPro IPR039261 Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
151 253 SUPERFAMILY SSF63380 Riboflavin synthase domain-like
151 253 InterPro IPR017938 Riboflavin synthase-like beta-barrel
150 388 CDD cd06184 flavohem_like_fad_nad_binding
152 254 FunFam G3DSA:2.40.30.10:FF:000034 Flavohemoprotein

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA2664
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.859

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

59 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
AZI
2VHB
P04252 42.0 Da LogP 0.87 TPSA 58.7 ✓ Ro5 Alert [N-]=[N+]=[N-]
DGG
1CQX
P39662 735.0 Da LogP 9.75 TPSA 148.8 2 viol. ✓ Clean CCCCCCCCCCCCCCCC(=O)OC[C@H](CO[P@@](=O)(O)OC[C@…
ECN
4G1B
A6ZUP2 381.7 Da LogP 5.80 TPSA 27.1 1 viol. ✓ Clean c1cc(ccc1COC(Cn2ccnc2)c3ccc(cc3Cl)Cl)Cl
FDA
1TVC
P22868 787.6 Da LogP -1.75 TPSA 363.3 3 viol. ✓ Clean Cc1cc2c(cc1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C…
FES
1JQ4
P22868 175.8 Da LogP 1.29 TPSA 0.0 ✓ Ro5 ✓ Clean S1[Fe]S[Fe]1
KKK
3OZW
P39662 531.4 Da LogP 4.21 TPSA 69.1 1 viol. Alert CC(=O)N1CCN(CC1)c2ccc(cc2)OC[C@H]3CO[C@](O3)(Cn…
NO2
4G1V
A6ZUP2 46.0 Da LogP 0.25 TPSA 52.5 ✓ Ro5 ✓ Clean N(=O)[O-]
OXY
3S1I
B3DUZ7 32.0 Da LogP 0.07 TPSA 34.1 ✓ Ro5 ✓ Clean O=O
X89
3OZU
P39662 416.1 Da LogP 6.45 TPSA 27.1 1 viol. ✓ Clean c1cc(c(cc1Cl)Cl)CO[C@@H](Cn2ccnc2)c3ccc(cc3Cl)Cl

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.