Protein profile

PA2795

tRNA-dihydrouridine synthase A

Genome: NC_002516.2

Gene: PA2795 dusA Structure source: AlphaFold UniProt Q9I048
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Amino acids 332
Annotations 10
Features 19
PDB binders 0
Druggability 0.672

Overview

Basic information about this protein and its source genome.

Accession
PA2795
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA2795 dusA
Status
annotated
Amino acids
332
Structure source
AlphaFold
EC
1.3.1.- 1.3.1.91
GO
GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2. GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes. GO:0000049 Binding to a transfer RNA. GO:0102264 Catalysis of the reaction: a 5,6-dihydrouracil20 in tRNA + NAD(P) = H+ + a uracil20 in tRNA + NAD(P)H. GO:0102266 Catalysis of the reaction: a 5,6-dihydrouracil20a in tRNA + NAD(P) = H+ + a uracil20a in tRNA + NAD(P)H. GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
32.941
Human E-value
1.66e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.672
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MRPEPTNAPAALSRRFSVAPMMDWTDRHCRFFLRQLSRHTLLYTEMVTTGALLHGDRQRFLRYDECEHPLALQLGGSVPAELAACARLAEEAGYDEVNLNVGCPSDRVQHNMIGACLMGHPALVADCVKAMLDAVEIAVTVKHRIGINGRDSYAELCDFVGQVREAGCRSFTVHARIAILEGLSPKENREVPPLRYEVAAQLKKDFPDLEIVLNGGIKTLEACREHLQTFDGVMLGREAYHNPYLLAAVDSQLFGSEAPPLSRSEALLRLRPYIERHQAEGGAMHHVTRHILGLAQGFPGSRRFRQLLSVDVHKAADPLRVFDQALELLAGR

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 EC 8 GO

Enzyme Commission (EC)

2

Gene Ontology (GO)

8
  • GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
  • GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
  • GO:0000049 Binding to a transfer RNA.
  • GO:0102264 Catalysis of the reaction: a 5,6-dihydrouracil20 in tRNA + NAD(P) = H+ + a uracil20 in tRNA + NAD(P)H.
  • GO:0102266 Catalysis of the reaction: a 5,6-dihydrouracil20a in tRNA + NAD(P) = H+ + a uracil20a in tRNA + NAD(P)H.
  • GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
  • GO:0002943 The process whereby a uridine in a transfer RNA is converted to dihydrouridine.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
258 331 Gene3D G3DSA:1.20.120.1460 -
18 312 Pfam PF01207 Dihydrouridine synthase (Dus)
18 312 InterPro IPR035587 DUS-like, FMN-binding domain
7 329 PANTHER PTHR42907 FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEIN
7 329 InterPro IPR004653 tRNA-dihydrouridine(20/20a) synthase
10 331 Hamap MF_02041 tRNA-dihydrouridine(20/20a) synthase [dusA].
10 331 InterPro IPR004653 tRNA-dihydrouridine(20/20a) synthase
12 254 Gene3D G3DSA:3.20.20.70 Aldolase class I
12 254 InterPro IPR013785 Aldolase-type TIM barrel
13 254 FunFam G3DSA:3.20.20.70:FF:000083 tRNA-dihydrouridine(20/20a) synthase
14 329 NCBIfam TIGR00742 tRNA dihydrouridine(20/20a) synthase DusA
14 329 InterPro IPR004653 tRNA-dihydrouridine(20/20a) synthase
15 254 CDD cd02801 DUS_like_FMN
15 254 InterPro IPR035587 DUS-like, FMN-binding domain
5 330 PIRSF PIRSF006621 Dus
5 330 InterPro IPR001269 tRNA-dihydrouridine synthase
97 115 ProSitePatterns PS01136 Uncharacterized protein family UPF0034 signature.
97 115 InterPro IPR018517 tRNA-dihydrouridine synthase, conserved site
15 325 SUPERFAMILY SSF51395 FMN-linked oxidoreductases

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA2795
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.672