Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA2830
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA2830 htpX
Status: annotated
Amino acids: 291
Structure source: AlphaFold

EC

3.4.24.-

GO

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. GO:0008270 Binding to a zinc ion (Zn). GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.707

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

MMRILLFLATNLAVLVIASITLKLLGVDRFTGQNYGSLLVFCAVFGFAGSLVSLFISKWMAKMSTGTEVISQPRTRHEQWLLQTVEELSREAGIKMPEVGIFPAYEANAFATGWNKNDALVAVSQGLLERFSPDEVKAVLAHEIGHVANGDMVTLALIQGVVNTFVMFFARIFGNFVDKAILKNEDGPGIGYFVATIFAELVLGILASIIVMWFSRRREFRADAAGAHLAGTGAMIAALQRLRSEQGVPVQMPDTLNAFGINGGLKHGLAGLLMSHPPLEDRIEALRASAR

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

3.4.24.-

Gene Ontology (GO)

4

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.
GO:0008270 Binding to a zinc ion (Zn).
GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records

Show feature table

Start	End	DB	Term	Name
46	287	CDD	cd07335	M48B_HtpX_like
20	34	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
2	290	PANTHER	PTHR43221	PROTEASE HTPX
152	174	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
77	288	Pfam	PF01435	Peptidase family M48
77	288	InterPro	IPR001915	Peptidase M48
1	289	Hamap	MF_00188	Protease HtpX [htpX].
1	289	InterPro	IPR022919	Peptidase M48, protease HtpX, putative
1	19	Phobius	SIGNAL_PEPTIDE	Signal peptide region
57	151	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
189	211	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
1	3	Phobius	SIGNAL_PEPTIDE_N_REGION	N-terminal region of a signal peptide.
152	170	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
171	189	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
37	56	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
215	291	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
190	214	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
5	27	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
16	19	Phobius	SIGNAL_PEPTIDE_C_REGION	C-terminal region of a signal peptide.
67	160	Gene3D	G3DSA:3.30.2010.10	Metalloproteases ("zincins"), catalytic domain
35	56	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
4	15	Phobius	SIGNAL_PEPTIDE_H_REGION	Hydrophobic region of a signal peptide.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

Loading 3D structure...

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA2830	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
4				0.502
3				0.475
8				0.256

PA2830