Protein profile

PA3004

S-methyl-5'-thioinosine phosphorylase

Genome: NC_002516.2

Gene: PA3004 Structure source: Experimental + AlphaFold UniProt Q9HZK1
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Amino acids 245
Annotations 8
Features 15
PDB binders 16
Druggability 0.908

Overview

Basic information about this protein and its source genome.

Accession
PA3004
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3004
Status
annotated
Amino acids
245
Structure source
Experimental + AlphaFold
EC
2.4.2.44
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0017061 Catalysis of the reaction: 5'-methylthioadenosine + phosphate = adenine + 5-methylthio-D-ribose 1-phosphate. GO:0019509 OBSOLETE. The generation of L-methionine (2-amino-4-(methylthio)butanoic acid) from methylthioadenosine. GO:0006166 Any process which produces a purine nucleoside from derivatives of it, without de novo synthesis. GO:0016763 Catalysis of the transfer of a pentosyl group from one compound (donor) to another (acceptor). GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
40.385
Human E-value
2.25e-07
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.908
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0017061 Catalysis of the reaction: 5'-methylthioadenosine + phosphate = adenine + 5-methylthio-D-ribose 1-phosphate.
  • GO:0019509 OBSOLETE. The generation of L-methionine (2-amino-4-(methylthio)butanoic acid) from methylthioadenosine.
  • GO:0006166 Any process which produces a purine nucleoside from derivatives of it, without de novo synthesis.
  • GO:0016763 Catalysis of the transfer of a pentosyl group from one compound (donor) to another (acceptor).
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
  • GO:0009116 The chemical reactions and pathways involving a nucleoside, a nucleobase linked to either beta-D-ribofuranose (a ribonucleoside) or 2-deoxy-beta-D-ribofuranose, (a deoxyribonucleoside), e.g. adenosine, guanosine, inosine, cytidine, uridine and deoxyadenosine, deoxyguanosine, deoxycytidine and thymidine (= deoxythymidine).

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
50 90 ProSitePatterns PS01240 Purine and other phosphorylases family 2 signature.
50 90 InterPro IPR018099 Purine phosphorylase, family 2, conserved site
1 245 Gene3D G3DSA:3.40.50.1580 Nucleoside phosphorylase domain
1 245 InterPro IPR035994 Nucleoside phosphorylase superfamily
4 243 Pfam PF01048 Phosphorylase superfamily
4 243 InterPro IPR000845 Nucleoside phosphorylase domain
5 243 CDD cd09010 MTAP_SsMTAPII_like_MTIP
5 243 InterPro IPR010044 Methylthioadenosine phosphorylase (MTAP)
2 245 Hamap MF_01963 S-methyl-5'-thioadenosine phosphorylase [mtnP].
2 245 InterPro IPR010044 Methylthioadenosine phosphorylase (MTAP)
4 243 SUPERFAMILY SSF53167 Purine and uridine phosphorylases
4 243 InterPro IPR035994 Nucleoside phosphorylase superfamily
4 243 PANTHER PTHR42679 S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE
4 243 InterPro IPR010044 Methylthioadenosine phosphorylase (MTAP)
4 243 NCBIfam TIGR01694 S-methyl-5'-thioadenosine phosphorylase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 3OZB
X-ray 2.80 Å A,B,C,D,E,F
100.0% 1-245
PDBe RCSB PDBj File
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AlphaFold PA3004
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.908

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 6.39 0.321
2 5.44 0.258
3 1.29 0.014
4 0.52 0.0

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

154 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Show only:
Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
HPA
3OZB 2.80 Å Open crystal
136.1 Da LogP -0.35 TPSA 74.4 ✓ Ro5 ✓ Clean c1[nH]c2c(n1)N=CNC2=O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.