Protein profile

PA3096

type II secretion system protein L

Genome: NC_002516.2

Gene: PA3096 xcpY Structure source: Experimental + AlphaFold UniProt P25060
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Amino acids 382
Annotations 4
Features 15
PDB binders 0
Druggability 0.926

Overview

Basic information about this protein and its source genome.

Accession
PA3096
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3096 xcpY
Status
annotated
Amino acids
382
Structure source
Experimental + AlphaFold
GO
GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.926
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
297 382 Gene3D G3DSA:3.30.1360.100 General secretion pathway protein M, EpsM
35 382 PIRSF PIRSF015761 Protein_L
35 382 InterPro IPR007812 Type II secretion system protein GspL
226 381 Pfam PF12693 GspL periplasmic domain
226 381 InterPro IPR025691 GspL periplasmic domain
6 163 Gene3D G3DSA:3.30.420.380 -
235 255 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
7 222 Pfam PF05134 Type II secretion system (T2SS), protein L
7 222 InterPro IPR024230 GspL, cytoplasmic actin-ATPase-like domain
256 382 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
17 376 NCBIfam TIGR01709 type II secretion system protein GspL
17 376 InterPro IPR007812 Type II secretion system protein GspL
6 139 SUPERFAMILY SSF53067 Actin-like ATPase domain
6 139 InterPro IPR043129 ATPase, nucleotide binding domain
1 234 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

2 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 6GHU
X-ray 2.00 Å A,B
20.4% 304-381
PDBe RCSB PDBj File
Viewing
PDB 5N7L
X-ray 2.50 Å A
20.9% 303-382
PDBe RCSB PDBj File
Loaded
AlphaFold PA3096
AlphaFold — — full sequence — Loaded
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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.926
2 0.315