Protein profile

PA3099

type II secretion system protein I

Genome: NC_002516.2

Gene: xcpV PA3099 pddC Structure source: Experimental + AlphaFold UniProt Q00516
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Amino acids 129
Annotations 3
Features 23
PDB binders 0
Druggability 0.729

Overview

Basic information about this protein and its source genome.

Accession
PA3099
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
xcpV PA3099 pddC
Status
annotated
Amino acids
129
Structure source
Experimental + AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.729
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MKRARGFTLLEVLVALAIFAMVAASVLSASARSLQNASRLEDKTLAMWIADNRLNELQLEQTPPSSGRNQGELEFAGRRWEWRTQVDSTAEQDMRRVIVWVAAKPLGRERGSIEERAAARLVGFLGSQP

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.

Sequence Features

Domain/signature hits from InterPro and related databases.

23 records
Show feature table
Start End DB Term Name
21 28 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 123 PANTHER PTHR38779 TYPE II SECRETION SYSTEM PROTEIN I-RELATED
1 123 InterPro IPR010052 Type II secretion system protein GspI
9 20 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 27 Pfam PF07963 Prokaryotic N-terminal methylation motif
1 27 InterPro IPR012902 Prokaryotic N-terminal methylation site
1 8 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
33 126 Gene3D G3DSA:3.30.1300.30 -
6 105 NCBIfam TIGR01707 type II secretion system minor pseudopilin GspI
6 105 InterPro IPR010052 Type II secretion system protein GspI
29 129 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 24 SignalP_EUK SignalP-TM SignalP-TM
1 31 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
5 26 NCBIfam TIGR02532 prepilin-type N-terminal cleavage/methylation domain
5 26 InterPro IPR012902 Prokaryotic N-terminal methylation site
1 28 Phobius SIGNAL_PEPTIDE Signal peptide region
38 124 SUPERFAMILY SSF54523 Pili subunits
38 124 InterPro IPR045584 Pilin-like
5 25 ProSitePatterns PS00409 Prokaryotic N-terminal methylation site.
5 25 InterPro IPR012902 Prokaryotic N-terminal methylation site
40 125 Pfam PF02501 Type II secretion system (T2SS), protein I
40 125 InterPro IPR003413 Type II secretion system protein GspI, C-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

3 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 5BW0
X-ray 2.00 Å B,D,F,H
72.9% 33-126
PDBe RCSB PDBj File
Viewing
PDB 5VTM
X-ray 2.04 Å V
71.3% 38-129
PDBe RCSB PDBj File
Loaded
PDB 6UTU
X-ray 2.85 Å A,D,G
71.3% 38-129
PDBe RCSB PDBj File
Loaded
AlphaFold PA3099
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.729