Protein profile

PA3114

tRNA pseudouridine synthase A

Genome: NC_002516.2

Gene: PA3114 truA hisT Structure source: AlphaFold UniProt O87016
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Amino acids 285
Annotations 7
Features 21
PDB binders 0
Druggability 0.506

Overview

Basic information about this protein and its source genome.

Accession
PA3114
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3114 truA hisT
Status
annotated
Amino acids
285
Structure source
AlphaFold
EC
5.4.99.12
GO
GO:0009982 Catalysis of the reaction: a uridine in RNA = a pseudouridine in RNA. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5. GO:0003723 Binding to an RNA molecule or a portion thereof. GO:0160147 Catalysis of the reaction: uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA. Modifies uridine(38), uridine(39) and/or uridine(40) in tRNA. GO:0031119 The intramolecular conversion of uridine to pseudouridine in a tRNA molecule. GO:0001522 The intramolecular conversion of uridine to pseudouridine within an RNA molecule. GO:0009451 The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
32.5
Human E-value
1.44e-09
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.506
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0009982 Catalysis of the reaction: a uridine in RNA = a pseudouridine in RNA. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.
  • GO:0003723 Binding to an RNA molecule or a portion thereof.
  • GO:0160147 Catalysis of the reaction: uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA. Modifies uridine(38), uridine(39) and/or uridine(40) in tRNA.
  • GO:0031119 The intramolecular conversion of uridine to pseudouridine in a tRNA molecule.
  • GO:0001522 The intramolecular conversion of uridine to pseudouridine within an RNA molecule.
  • GO:0009451 The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
22 261 CDD cd02570 PseudoU_synth_EcTruA
22 261 InterPro IPR001406 Pseudouridine synthase I, TruA
15 122 Gene3D G3DSA:3.30.70.580 -
15 122 InterPro IPR020094 Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal
18 261 Hamap MF_00171 tRNA pseudouridine synthase A [truA].
18 261 InterPro IPR001406 Pseudouridine synthase I, TruA
17 122 FunFam G3DSA:3.30.70.580:FF:000001 tRNA pseudouridine synthase A
18 275 SUPERFAMILY SSF55120 Pseudouridine synthase
18 275 InterPro IPR020103 Pseudouridine synthase, catalytic domain superfamily
17 277 PIRSF PIRSF001430 PSU-A
17 277 InterPro IPR001406 Pseudouridine synthase I, TruA
123 260 Gene3D G3DSA:3.30.70.660 -
123 260 InterPro IPR020095 Pseudouridine synthase I, TruA, C-terminal
160 262 Pfam PF01416 tRNA pseudouridine synthase
160 262 InterPro IPR020097 Pseudouridine synthase I, TruA, alpha/beta domain
25 120 Pfam PF01416 tRNA pseudouridine synthase
25 120 InterPro IPR020097 Pseudouridine synthase I, TruA, alpha/beta domain
19 256 NCBIfam TIGR00071 tRNA pseudouridine(38-40) synthase TruA
19 256 InterPro IPR001406 Pseudouridine synthase I, TruA
17 265 PANTHER PTHR11142 PSEUDOURIDYLATE SYNTHASE
17 265 InterPro IPR001406 Pseudouridine synthase I, TruA

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3114
AlphaFold — — full sequence — Viewing
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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
5 0.506
1 0.211