Protein profile

PA3129

tRNA-dihydrouridine synthase C

Genome: NC_002516.2

Gene: PA3129 dusC Structure source: AlphaFold UniProt Q9HZ95
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Amino acids 319
Annotations 8
Features 16
PDB binders 0
Druggability 0.32

Overview

Basic information about this protein and its source genome.

Accession
PA3129
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3129 dusC
Status
annotated
Amino acids
319
Structure source
AlphaFold
EC
1.3.1.-
GO
GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2. GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes. GO:0000049 Binding to a transfer RNA. GO:0102262 Catalysis of the reaction: a 5,6-dihydrouracil16 in tRNA + NAD(P) = H+ + a uracil16 in tRNA + NAD(P)H. GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H. GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
33.871
Human E-value
6.24e-10
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.32
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
  • GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
  • GO:0000049 Binding to a transfer RNA.
  • GO:0102262 Catalysis of the reaction: a 5,6-dihydrouracil16 in tRNA + NAD(P) = H+ + a uracil16 in tRNA + NAD(P)H.
  • GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
  • GO:0002943 The process whereby a uridine in a transfer RNA is converted to dihydrouridine.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
5 249 Pfam PF01207 Dihydrouridine synthase (Dus)
5 249 InterPro IPR035587 DUS-like, FMN-binding domain
2 311 SUPERFAMILY SSF51395 FMN-linked oxidoreductases
3 241 CDD cd02801 DUS_like_FMN
3 241 InterPro IPR035587 DUS-like, FMN-binding domain
1 319 PIRSF PIRSF006621 Dus
1 319 InterPro IPR001269 tRNA-dihydrouridine synthase
247 316 Gene3D G3DSA:1.20.225.30 -
247 316 InterPro IPR042270 tRNA-dihydrouridine(16) synthase, C-terminal
4 300 PANTHER PTHR11082 TRNA-DIHYDROURIDINE SYNTHASE
1 239 Gene3D G3DSA:3.20.20.70 Aldolase class I
1 239 InterPro IPR013785 Aldolase-type TIM barrel
8 314 Hamap MF_02043 tRNA-dihydrouridine(16) synthase [dusC].
8 314 InterPro IPR032886 tRNA-dihydrouridine(16) synthase
92 110 ProSitePatterns PS01136 Uncharacterized protein family UPF0034 signature.
92 110 InterPro IPR018517 tRNA-dihydrouridine synthase, conserved site

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3129
AlphaFold — — full sequence — Viewing
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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.32
1 0.238
2 0.223