Protein profile

PA3306

hypothetical protein

Genome: NC_002516.2

Gene: PA3306 Structure source: AlphaFold UniProt Q9HYT8
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Amino acids 200
Annotations 7
Features 10
PDB binders 2
Druggability 0.789

Overview

Basic information about this protein and its source genome.

Accession
PA3306
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3306
Status
annotated
Amino acids
200
Structure source
AlphaFold
GO
GO:0035516 Catalysis of the reaction: a methylated nucleobase within DNA + 2-oxoglutarate + O2 = a nucleobase within DNA + formaldehyde + succinate + CO2. Catalyzes oxidative demethylation of the DNA base lesions N1- methyladenine, N3-methylcytosine, N1-methylguanine, and N3- methylthymine. Can also act of RNA. GO:0051747 Catalysis of the reaction: methyl-dCpdG DNA + H2O = dCpdG DNA + methanol. This reaction is the hydrolytic removal of the methyl group on the 5 position of cytosine in DNA. GO:0008198 Binding to a ferrous iron ion, Fe(II). GO:0006307 The repair of alkylation damage in DNA, e.g. the removal of a non-physiological alkyl group from a nucleobase. This is usually mediated by DNA alkyltransferases. GO:0006281 The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway. GO:0051213 Catalysis of the incorporation of both atoms of molecular oxygen (O2) into the substrate.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
46.094
Human E-value
4.34e-33
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.789
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0035516 Catalysis of the reaction: a methylated nucleobase within DNA + 2-oxoglutarate + O2 = a nucleobase within DNA + formaldehyde + succinate + CO2. Catalyzes oxidative demethylation of the DNA base lesions N1- methyladenine, N3-methylcytosine, N1-methylguanine, and N3- methylthymine. Can also act of RNA.
  • GO:0051747 Catalysis of the reaction: methyl-dCpdG DNA + H2O = dCpdG DNA + methanol. This reaction is the hydrolytic removal of the methyl group on the 5 position of cytosine in DNA.
  • GO:0008198 Binding to a ferrous iron ion, Fe(II).
  • GO:0006307 The repair of alkylation damage in DNA, e.g. the removal of a non-physiological alkyl group from a nucleobase. This is usually mediated by DNA alkyltransferases.
  • GO:0006281 The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
  • GO:0051213 Catalysis of the incorporation of both atoms of molecular oxygen (O2) into the substrate.
  • GO:0035552 OBSOLETE. Removal of the methyl group from one or more nucleotides within a single-stranded DNA molecule involving the oxidation (i.e. electron loss) of one or more atoms.

Sequence Features

Domain/signature hits from InterPro and related databases.

10 records
Show feature table
Start End DB Term Name
16 194 Pfam PF13532 2OG-Fe(II) oxygenase superfamily
16 194 InterPro IPR027450 Alpha-ketoglutarate-dependent dioxygenase AlkB-like
99 197 ProSiteProfiles PS51471 Fe(2+) 2-oxoglutarate dioxygenase domain profile.
99 197 InterPro IPR005123 Oxoglutarate/iron-dependent dioxygenase
16 199 PANTHER PTHR31212 ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3
16 199 InterPro IPR032854 Alkylated DNA repair protein alkB homologue 3
8 198 SUPERFAMILY SSF51197 Clavaminate synthase-like
8 199 Gene3D G3DSA:2.60.120.590 -
8 199 InterPro IPR037151 Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily
6 197 FunFam G3DSA:2.60.120.590:FF:000004 DNA oxidative demethylase ALKBH2

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3306
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.789
3 0.353
5 0.211

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

74 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
AKG
3BU0
Q6NS38 146.1 Da LogP -0.50 TPSA 91.7 ✓ Ro5 ✓ Clean C(CC(=O)O)C(=O)C(=O)O
XL3
3BTY
Q6NS38 76.2 Da LogP 1.33 TPSA 0.0 ✓ Ro5 ✓ Clean CCCS

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.