Protein profile

PA3321

transcriptional regulator

Genome: NC_002516.2

Gene: PA3321 Structure source: AlphaFold UniProt Q9HYS4
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Amino acids 304
Annotations 3
Features 21
PDB binders 4
Druggability 0.872

Overview

Basic information about this protein and its source genome.

Accession
PA3321
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3321
Status
annotated
Amino acids
304
Structure source
AlphaFold
GO
GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.872
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
27 38 PRINTS PR00039 LysR bacterial regulatory protein HTH signature
27 38 InterPro IPR000847 Transcription regulator HTH, LysR
38 48 PRINTS PR00039 LysR bacterial regulatory protein HTH signature
38 48 InterPro IPR000847 Transcription regulator HTH, LysR
48 59 PRINTS PR00039 LysR bacterial regulatory protein HTH signature
48 59 InterPro IPR000847 Transcription regulator HTH, LysR
95 296 Gene3D G3DSA:3.40.190.10 -
99 301 Pfam PF03466 LysR substrate binding domain
99 301 InterPro IPR005119 LysR, substrate-binding
93 299 SUPERFAMILY SSF53850 Periplasmic binding protein-like II
1 302 PANTHER PTHR30118 HTH-TYPE TRANSCRIPTIONAL REGULATOR LEUO-RELATED
3 88 Gene3D G3DSA:1.10.10.10 -
3 88 InterPro IPR036388 Winged helix-like DNA-binding domain superfamily
101 300 CDD cd08465 PBP2_ToxR
12 68 Pfam PF00126 Bacterial regulatory helix-turn-helix protein, lysR family
12 68 InterPro IPR000847 Transcription regulator HTH, LysR
7 87 SUPERFAMILY SSF46785 Winged helix DNA-binding domain
7 87 InterPro IPR036390 Winged helix DNA-binding domain superfamily
174 272 Gene3D G3DSA:3.40.190.10 -
10 67 ProSiteProfiles PS50931 LysR-type HTH domain profile.
10 67 InterPro IPR000847 Transcription regulator HTH, LysR

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3321
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.872
2 0.621

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
PCI
4RPN
P52679 266.3 Da LogP 4.66 TPSA 20.2 ✓ Ro5 ✓ Clean c1(c(c(c(c(c1Cl)Cl)Cl)Cl)Cl)O
PEU
2Y7P
Q7WT50 1221.5 Da LogP 0.06 TPSA 269.4 2 viol. ✓ Clean COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO…
SAL
2Y7K
Q7WT50 138.1 Da LogP 1.09 TPSA 57.5 ✓ Ro5 ✓ Clean c1ccc(c(c1)C(=O)O)O
T6C
4RPO
P52679 197.4 Da LogP 3.35 TPSA 20.2 ✓ Ro5 ✓ Clean c1c(cc(c(c1Cl)O)Cl)Cl

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.