Protein profile

PA3339

patatin-like protein

Genome: NC_002516.2

Gene: plpD PA3339 Structure source: Experimental + AlphaFold UniProt Q9HYQ6
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Amino acids 728
Annotations 7
Features 23
PDB binders 1
Druggability 0.953

Overview

Basic information about this protein and its source genome.

Accession
PA3339
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
plpD PA3339
Status
annotated
Amino acids
728
Structure source
Experimental + AlphaFold
GO
GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures. GO:0005576 The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite. GO:0016298 Catalysis of the hydrolysis of a lipid. GO:0016042 The chemical reactions and pathways resulting in the breakdown of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. GO:0046819 The process in which proteins mediate their own secretion across the outer membrane through a beta-barrel pore structure formed by the C-terminal domain of the protein precursor. Following passage across the outer membrane, the mature protein is released from the pore by an autocatalytic activity. Proteins secreted by the Type V system are first translocated across the plasma membrane by the Sec pathway. GO:0006629 The chemical reactions and pathways involving lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. Includes fatty acids; neutral fats, other fatty-acid esters, and soaps; long-chain (fatty) alcohols and waxes; sphingoids and other long-chain bases; glycolipids, phospholipids and sphingolipids; and carotenes, polyprenols, sterols, terpenes and other isoprenoids.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
31.878
Human E-value
2.62e-26
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
OuterMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.953
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.
  • GO:0005576 The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
  • GO:0016298 Catalysis of the hydrolysis of a lipid.
  • GO:0016042 The chemical reactions and pathways resulting in the breakdown of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
  • GO:0046819 The process in which proteins mediate their own secretion across the outer membrane through a beta-barrel pore structure formed by the C-terminal domain of the protein precursor. Following passage across the outer membrane, the mature protein is released from the pore by an autocatalytic activity. Proteins secreted by the Type V system are first translocated across the plasma membrane by the Sec pathway.
  • GO:0006629 The chemical reactions and pathways involving lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. Includes fatty acids; neutral fats, other fatty-acid esters, and soaps; long-chain (fatty) alcohols and waxes; sphingoids and other long-chain bases; glycolipids, phospholipids and sphingolipids; and carotenes, polyprenols, sterols, terpenes and other isoprenoids.
  • GO:0019867 The external membrane of Gram-negative bacteria or certain organelles such as mitochondria and chloroplasts; freely permeable to most ions and metabolites.

Sequence Features

Domain/signature hits from InterPro and related databases.

23 records
Show feature table
Start End DB Term Name
329 402 Gene3D G3DSA:3.10.20.310 membrane protein fhac
4 14 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 21 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
449 728 Pfam PF01103 Omp85 superfamily domain
449 728 InterPro IPR000184 Bacterial surface antigen (D15)
411 728 Gene3D G3DSA:2.40.160.50 membrane protein fhac: a member of the omp85/tpsb transporter family
1 18 SignalP_EUK SignalP-noTM SignalP-noTM
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
15 18 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
27 219 Pfam PF01734 Patatin-like phospholipase
27 219 InterPro IPR002641 Patatin-like phospholipase domain
333 393 Pfam PF07244 Surface antigen variable number repeat
333 393 InterPro IPR010827 POTRA domain, BamA/TamA-like
19 728 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 18 Phobius SIGNAL_PEPTIDE Signal peptide region
27 220 ProSiteProfiles PS51635 Patatin-like phospholipase (PNPLA) domain profile.
27 220 InterPro IPR002641 Patatin-like phospholipase domain
22 317 PANTHER PTHR14226 NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER
17 302 SUPERFAMILY SSF52151 FabD/lysophospholipase-like
17 302 InterPro IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase
25 222 CDD cd07205 Pat_PNPLA6_PNPLA7_NTE1_like
15 126 Gene3D G3DSA:3.40.1090.10 Cytosolic phospholipase A2 catalytic domain
133 269 Gene3D G3DSA:3.40.1090.10 Cytosolic phospholipase A2 catalytic domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

2 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 5FYA
X-ray 2.14 Å A,B
43.1% 20-333
PDBe RCSB PDBj File
Viewing
PDB 5FQU
X-ray 2.74 Å A,B
43.1% 20-333
PDBe RCSB PDBj File
Loaded
AlphaFold PA3339
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.953

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 34.57 0.951
2 9.5 0.511

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

1 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Show only:
Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
AZI
5FQU 2.74 Å Open crystal
42.0 Da LogP 0.87 TPSA 58.7 ✓ Ro5 Alert [N-]=[N+]=[N-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.