Protein profile

PA3348

chemotaxis protein methyltransferase

Genome: NC_002516.2

Gene: PA3348 cheR1 Structure source: Experimental + AlphaFold UniProt O87131
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Amino acids 274
Annotations 5
Features 31
PDB binders 0
Druggability 0.697

Overview

Basic information about this protein and its source genome.

Accession
PA3348
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3348 cheR1
Status
annotated
Amino acids
274
Structure source
Experimental + AlphaFold
EC
2.1.1.80
GO
GO:0008276 Catalysis of the transfer of a methyl group (CH3-) to a protein. GO:0008983 Catalysis of the reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate 5-methyl ester; this reaction is the methylation of peptidyl-L-glutamate to form peptidyl-L-glutamate 5-methyl ester. GO:0032259 The process in which a methyl group is covalently attached to a molecule. GO:0008757 Catalysis of the transfer of a methyl group from S-adenosyl-L-methionine to a substrate.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.697
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MSAANADFELFRVFLEKTCGIVLGSNKQYLVSSRLNKLMEQQGIKSLGELVQRIQTQRGGLREMVVDAMTTNETLWFRDTYPFEVLKQRVLPELIKANGGQRLRIWSAACSSGQEPYSLSMAIDEFEKTNLGQLKAGVQIVATDLSGSMLTAAKAGEYDTLAMGRGLSPERLQRYFDAKGPGRWAVKPAIRSRVEFRALNLLDSYASLGKFDMVFCRNVLIYFSAEVKRDILLRIHGTLKPGGYLFLGASEALNNLPDHYQMVQCSPGIIYRAK

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0008276 Catalysis of the transfer of a methyl group (CH3-) to a protein.
  • GO:0008983 Catalysis of the reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate 5-methyl ester; this reaction is the methylation of peptidyl-L-glutamate to form peptidyl-L-glutamate 5-methyl ester.
  • GO:0032259 The process in which a methyl group is covalently attached to a molecule.
  • GO:0008757 Catalysis of the transfer of a methyl group from S-adenosyl-L-methionine to a substrate.

Sequence Features

Domain/signature hits from InterPro and related databases.

31 records
Show feature table
Start End DB Term Name
27 40 PRINTS PR00996 Glutamate methyltransferase family signature
27 40 InterPro IPR000780 MCP methyltransferase, CheR-type
104 124 PRINTS PR00996 Glutamate methyltransferase family signature
104 124 InterPro IPR000780 MCP methyltransferase, CheR-type
230 253 PRINTS PR00996 Glutamate methyltransferase family signature
230 253 InterPro IPR000780 MCP methyltransferase, CheR-type
65 77 PRINTS PR00996 Glutamate methyltransferase family signature
65 77 InterPro IPR000780 MCP methyltransferase, CheR-type
142 158 PRINTS PR00996 Glutamate methyltransferase family signature
142 158 InterPro IPR000780 MCP methyltransferase, CheR-type
211 225 PRINTS PR00996 Glutamate methyltransferase family signature
211 225 InterPro IPR000780 MCP methyltransferase, CheR-type
72 267 Pfam PF01739 CheR methyltransferase, SAM binding domain
72 267 InterPro IPR022642 MCP methyltransferase, CheR-type, SAM-binding domain, C-terminal
7 264 PANTHER PTHR24422 CHEMOTAXIS PROTEIN METHYLTRANSFERASE
1 274 PIRSF PIRSF000410 CheR
1 274 InterPro IPR026024 Chemotaxis protein methyltransferase CheR
6 56 Pfam PF03705 CheR methyltransferase, all-alpha domain
6 56 InterPro IPR022641 Chemotaxis receptor methyltransferase CheR, N-terminal
1 274 ProSiteProfiles PS50123 CheR-type methyltransferase domain profile.
1 274 InterPro IPR000780 MCP methyltransferase, CheR-type
4 71 SUPERFAMILY SSF47757 Chemotaxis receptor methyltransferase CheR, N-terminal domain
1 70 Gene3D G3DSA:1.10.155.10 -
1 70 InterPro IPR036804 Chemotaxis receptor methyltransferase CheR, N-terminal domain superfamily
5 271 SMART SM00138 methyl_trans_3
5 271 InterPro IPR000780 MCP methyltransferase, CheR-type
191 247 CDD cd02440 AdoMet_MTases
72 265 SUPERFAMILY SSF53335 S-adenosyl-L-methionine-dependent methyltransferases
72 265 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
71 272 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
71 272 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

4 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 5XLY
X-ray 1.76 Å A
100.0% 1-274
PDBe RCSB PDBj File
Viewing
PDB 5XLX
X-ray 1.97 Å A,B,C,D
100.0% 1-274
PDBe RCSB PDBj File
Loaded
PDB 5Y4R
X-ray 2.30 Å A,B
99.6% 2-274
PDBe RCSB PDBj File
Loaded
PDB 5Y4S
X-ray 3.40 Å A,B,C,D,E,F,G,H,I,J
99.6% 2-274
PDBe RCSB PDBj File
Loaded
AlphaFold PA3348
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 4.3 0.178
2 3.41 0.122