Protein profile

PA3388

S-adenosylmethionine-dependent methyltransferase RcsF

Genome: NC_002516.2

Gene: PA3388 rcsF Structure source: Experimental + AlphaFold UniProt Q9RPT0
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Amino acids 231
Annotations 3
Features 20
PDB binders 0
Druggability 0.783

Overview

Basic information about this protein and its source genome.

Accession
PA3388
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3388 rcsF
Status
annotated
Amino acids
231
Structure source
Experimental + AlphaFold
EC
2.1.1.-
GO
GO:0089715 Catalysis of the reaction: S-adenosyl-L-methionine + tRNA containing N6-threonylcarbamoyladenosine at position 37 = S-adenosyl-L-homocysteine + tRNA containing N6-methylthreonylcarbamoyladenosine at position 37. GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
46.259
Human E-value
6.84e-38
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.783
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 2 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

2
  • GO:0089715 Catalysis of the reaction: S-adenosyl-L-methionine + tRNA containing N6-threonylcarbamoyladenosine at position 37 = S-adenosyl-L-homocysteine + tRNA containing N6-methylthreonylcarbamoyladenosine at position 37.
  • GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records
Show feature table
Start End DB Term Name
4 143 NCBIfam TIGR00104 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO
4 143 InterPro IPR023370 TrmO-like, N-terminal domain
7 133 CDD cd09281 UPF0066
7 133 InterPro IPR023370 TrmO-like, N-terminal domain
5 142 ProSiteProfiles PS51668 TsaA-like domain profile.
5 142 InterPro IPR023370 TrmO-like, N-terminal domain
21 138 Pfam PF01980 tRNA-methyltransferase O
21 138 InterPro IPR023370 TrmO-like, N-terminal domain
137 221 Gene3D G3DSA:3.30.2310.10 -
1 136 Gene3D G3DSA:2.40.30.70 -
1 136 InterPro IPR036414 YaeB, N-terminal domain superfamily
155 216 Pfam PF18389 TrmO C-terminal domain
155 216 InterPro IPR041369 TrmO, C-terminal
2 218 SUPERFAMILY SSF118196 YaeB-like
2 218 InterPro IPR036413 YaeB-like superfamily
86 97 ProSitePatterns PS01318 TsaA-like domain signature.
86 97 InterPro IPR023368 Uncharacterised protein family UPF0066, conserved site
1 136 FunFam G3DSA:2.40.30.70:FF:000008 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO
4 227 PANTHER PTHR12818 UNCHARACTERIZED
4 227 InterPro IPR040372 YaeB-like

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

3 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 7BTU
X-ray 1.91 Å A,B
100.0% 1-231
PDBe RCSB PDBj File
Viewing
PDB 7BTZ
X-ray 2.40 Å A,B
100.0% 1-231
PDBe RCSB PDBj File
Loaded
PDB 7BU1
X-ray 2.49 Å A,B
100.0% 1-231
PDBe RCSB PDBj File
Loaded
AlphaFold PA3388
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.783
1 0.425

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 4.87 0.218
2 2.02 0.044