Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA3452
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA3452 mqoA mqo1
Status: annotated
Amino acids: 523
Structure source: AlphaFold

EC

1.1.5.4

GO

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0008924 Catalysis of the reaction: (S)-malate + a quinone = oxaloacetate + a quinol. Vitamin K (menaquinone) and several other quinones can act as acceptors. GO:0006099 A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): N
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.731

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

MKKILLMLLCVSVLGCSKNSVESEKPVDVLLIGGGIMSATLGTYLNELEPGWTIEMVERLDKVAEESSNGWNNAGTGHSAFCELNYTSEAADGSMDISKAVAINENFEISKQFWAYQVDRKVLNDPKSFINNVPHMSFVWGDDNVAFLKKRHAALQHSSLFRGMEYSEDPEQIKQWVPLVMEGREPGQKIAATRMSIGTDVNFGEITRQLVGSLSAKDTFKLRLQHEVRDLKRNDDNTWTVTMADLANGDKETSVKARFVFIGAGGGALKLLQMSGIPEAEGYAGFPVGGSFLATTNPDVVKRHLAKVYGKASVGSPPMSVPHLDTRMIDGKPVLLFGPFATFSTKFLKNGSLWDLPGSVTSGNIGPMFNAGIDNFDLSQYLIGQLMLSQDDRMASLREYFPEARDEDWKLVQAGQRVQIIKKDAEKGGVLQFGTEVVTAADGSVAALLGASPGASTAAPIMLSVLEKAFKDKVATPEWQARLKEIVPSYGRKLNNDIELTNSTRAWSSERLQLIHVPVQPEA

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 3 GO

Enzyme Commission (EC)

1

1.1.5.4

Gene Ontology (GO)

3

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0008924 Catalysis of the reaction: (S)-malate + a quinone = oxaloacetate + a quinol. Vitamin K (menaquinone) and several other quinones can act as acceptors.
GO:0006099 A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

Sequence Features

Domain/signature hits from InterPro and related databases.

17 records

Show feature table

Start	End	DB	Term	Name
24	523	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1	16	ProSiteProfiles	PS51257	Prokaryotic membrane lipoprotein lipid attachment site profile.
1	3	Phobius	SIGNAL_PEPTIDE_N_REGION	N-terminal region of a signal peptide.
1	23	SignalP_GRAM_POSITIVE	SignalP-TM	SignalP-TM
1	23	SignalP_EUK	SignalP-noTM	SignalP-noTM
23	514	Hamap	MF_00212	Probable malate:quinone oxidoreductase [mqo].
23	514	InterPro	IPR006231	Malate:quinone-oxidoreductase
1	23	Phobius	SIGNAL_PEPTIDE	Signal peptide region
24	354	SUPERFAMILY	SSF51905	FAD/NAD(P)-binding domain
24	354	InterPro	IPR036188	FAD/NAD(P)-binding domain superfamily
16	23	Phobius	SIGNAL_PEPTIDE_C_REGION	C-terminal region of a signal peptide.
27	514	Pfam	PF06039	Malate:quinone oxidoreductase (Mqo)
27	514	InterPro	IPR006231	Malate:quinone-oxidoreductase
27	511	NCBIfam	TIGR01320	malate dehydrogenase (quinone)
27	511	InterPro	IPR006231	Malate:quinone-oxidoreductase
4	15	Phobius	SIGNAL_PEPTIDE_H_REGION	Hydrophobic region of a signal peptide.
22	469	PANTHER	PTHR43104	L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA3452	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.502

PA3452