Protein profile

PA3476

acyl-homoserine-lactone synthase

Genome: NC_002516.2

Gene: PA3476 vsmI rhlI Structure source: AlphaFold UniProt P54291
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Amino acids 201
Annotations 5
Features 25
PDB binders 4
Druggability 0.893

Overview

Basic information about this protein and its source genome.

Accession
PA3476
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3476 vsmI rhlI
Status
annotated
Amino acids
201
Structure source
AlphaFold
EC
2.3.1.184
GO
GO:0061579 Catalysis of the reaction: a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+. GO:0120218 A quorum sensing process that is modulated by some interaction with a host cell or organism. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016740 Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.893
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0061579 Catalysis of the reaction: a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+.
  • GO:0120218 A quorum sensing process that is modulated by some interaction with a host cell or organism.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016740 Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.

Sequence Features

Domain/signature hits from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
1 184 ProSiteProfiles PS51187 Autoinducer synthase family profile.
1 184 InterPro IPR001690 Autoinducer synthase
23 51 ProSitePatterns PS00949 Autoinducer synthase family signature.
23 51 InterPro IPR018311 Autoinducer synthesis, conserved site
5 186 Gene3D G3DSA:3.40.630.30 -
115 136 PRINTS PR01549 Autoinducer synthesis protein signature
115 136 InterPro IPR001690 Autoinducer synthase
97 109 PRINTS PR01549 Autoinducer synthesis protein signature
97 109 InterPro IPR001690 Autoinducer synthase
65 83 PRINTS PR01549 Autoinducer synthesis protein signature
65 83 InterPro IPR001690 Autoinducer synthase
137 158 PRINTS PR01549 Autoinducer synthesis protein signature
137 158 InterPro IPR001690 Autoinducer synthase
159 176 PRINTS PR01549 Autoinducer synthesis protein signature
159 176 InterPro IPR001690 Autoinducer synthase
19 38 PRINTS PR01549 Autoinducer synthesis protein signature
19 38 InterPro IPR001690 Autoinducer synthase
42 64 PRINTS PR01549 Autoinducer synthesis protein signature
42 64 InterPro IPR001690 Autoinducer synthase
1 201 PANTHER PTHR39322 ACYL-HOMOSERINE-LACTONE SYNTHASE
1 201 InterPro IPR001690 Autoinducer synthase
1 193 SUPERFAMILY SSF55729 Acyl-CoA N-acyltransferases (Nat)
1 193 InterPro IPR016181 Acyl-CoA N-acyltransferase
12 179 Pfam PF00765 Autoinducer synthase
12 179 InterPro IPR001690 Autoinducer synthase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3476
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.893

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
MTA
3P2H
Q4VSJ8 297.3 Da LogP -0.61 TPSA 119.3 ✓ Ro5 ✓ Clean CSC[C@@H]1[C@H]([C@H]([C@@H](O1)n2cnc3c2ncnc3N)…
NOO
3P2H
Q4VSJ8 237.3 Da LogP 3.10 TPSA 46.2 ✓ Ro5 ✓ Clean CCCCCCCC(=O)NC1=CC(=O)CCC1
REO
1K4J
P54656 250.2 Da LogP -1.55 TPSA 74.3 ✓ Ro5 ✓ Clean [O-][Re](=O)(=O)=O
U4Y
6WN0
Q6NCZ6 532.6 Da LogP -0.62 TPSA 205.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.