Protein profile

PA3478

rhamnosyltransferase subunit B

Genome: NC_002516.2

Gene: rhlB PA3478 Structure source: AlphaFold UniProt Q9HYD1
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Amino acids 426
Annotations 5
Features 10
PDB binders 4
Druggability 0.848

Overview

Basic information about this protein and its source genome.

Accession
PA3478
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
rhlB PA3478
Status
annotated
Amino acids
426
Structure source
AlphaFold
GO
GO:0016758 Catalysis of the transfer of a hexosyl group from one compound (donor) to another (acceptor). GO:0008194 Catalysis of the transfer of a glycosyl group from a UDP-sugar to a small hydrophobic molecule. GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. GO:0033072 The chemical reactions and pathways leading to the formation of vancomycin, (3S,6R,7R,11R,23S,26S,30aS,36R,38aR)-44-[2-O-(3-amino-2,3,6-trideoxy-3-C-methyl-alpha-L-lyxo-hexopyranosyl)-beta-D-glucopyranosyloxy]-3-(carbamoylmethyl)-10,19-dichloro-2,3,4,5,6,7,23,25,26,36,37,38,38a-tetradecahydro-7,22,28,30,32-pentahydroxy-6-(N-methyl-D-leucyl)-2,5,24,38,39-pentaoxo-1H,22H-23,36-(epiminomethano)-8,11:18,21-dietheno-13,16:31,35-di(metheno)[1,6,9]oxadiazacyclohexadecino[4,5-m][10,2,16]benzoxadiazacyclotetracosine-26-carboxylic acid, a complex glycopeptide from Streptomyces orientalis that inhibits a specific step in the synthesis of the peptidoglycan layer in Gram-positive bacteria. GO:0030259 OBSOLETE. Covalent attachment of a glycosyl residue to a lipid molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
26.316
Human E-value
1.62e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.848
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0016758 Catalysis of the transfer of a hexosyl group from one compound (donor) to another (acceptor).
  • GO:0008194 Catalysis of the transfer of a glycosyl group from a UDP-sugar to a small hydrophobic molecule.
  • GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0033072 The chemical reactions and pathways leading to the formation of vancomycin, (3S,6R,7R,11R,23S,26S,30aS,36R,38aR)-44-[2-O-(3-amino-2,3,6-trideoxy-3-C-methyl-alpha-L-lyxo-hexopyranosyl)-beta-D-glucopyranosyloxy]-3-(carbamoylmethyl)-10,19-dichloro-2,3,4,5,6,7,23,25,26,36,37,38,38a-tetradecahydro-7,22,28,30,32-pentahydroxy-6-(N-methyl-D-leucyl)-2,5,24,38,39-pentaoxo-1H,22H-23,36-(epiminomethano)-8,11:18,21-dietheno-13,16:31,35-di(metheno)[1,6,9]oxadiazacyclohexadecino[4,5-m][10,2,16]benzoxadiazacyclotetracosine-26-carboxylic acid, a complex glycopeptide from Streptomyces orientalis that inhibits a specific step in the synthesis of the peptidoglycan layer in Gram-positive bacteria.
  • GO:0030259 OBSOLETE. Covalent attachment of a glycosyl residue to a lipid molecule.

Sequence Features

Domain/signature hits from InterPro and related databases.

10 records
Show feature table
Start End DB Term Name
1 400 PANTHER PTHR48050 STEROL 3-BETA-GLUCOSYLTRANSFERASE
4 135 Pfam PF03033 Glycosyltransferase family 28 N-terminal domain
4 135 InterPro IPR004276 Glycosyltransferase family 28, N-terminal domain
4 228 Gene3D G3DSA:3.40.50.2000 Glycogen Phosphorylase B;
231 397 Pfam PF00201 UDP-glucoronosyl and UDP-glucosyl transferase
231 397 InterPro IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase
1 415 SUPERFAMILY SSF53756 UDP-Glycosyltransferase/glycogen phosphorylase
229 391 Gene3D G3DSA:3.40.50.2000 Glycogen Phosphorylase B;
1 412 CDD cd03784 GT1_Gtf-like
1 412 InterPro IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3478
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.848
2 0.729

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ERY
4M83
Q53685 733.9 Da LogP 1.79 TPSA 193.9 2 viol. ✓ Clean CC[C@@H]1[C@@]([C@@H]([C@H](C(=O)[C@@H](C[C@@](…
TYD
3WAG
Q76KZ6 402.2 Da LogP -1.28 TPSA 197.6 ✓ Ro5 ✓ Clean CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO[P@]…
U2F
3H4I
P96558 568.3 Da LogP -3.82 TPSA 276.8 3 viol. ✓ Clean C1=CN(C(=O)NC1=O)[C@H]2[C@@H]([C@@H]([C@H](O2)C…
UPG
5GL5
Q06321 566.3 Da LogP -4.79 TPSA 297.0 3 viol. ✓ Clean C1=CN(C(=O)NC1=O)[C@H]2[C@@H]([C@@H]([C@H](O2)C…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.