Protein profile

PA3528

ribonuclease T

Genome: NC_002516.2

Gene: rnt PA3528 Structure source: Experimental + AlphaFold UniProt Q9HY82
Export view
Amino acids 224
Annotations 10
Features 16
PDB binders 0
Druggability 0.89

Overview

Basic information about this protein and its source genome.

Accession
PA3528
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
rnt PA3528
Status
annotated
Amino acids
224
Structure source
Experimental + AlphaFold
EC
3.1.13.-
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0008408 Catalysis of the hydrolysis of ester linkages within nucleic acids by removing nucleotide residues from the 3' end. GO:0000287 Binding to a magnesium (Mg) ion. GO:0003676 Binding to a nucleic acid. GO:0016896 Catalysis of the hydrolysis of ester linkages within ribonucleic acids by removing nucleotide residues from the 3' or 5' end to yield 5' phosphomonoesters. GO:0045004 Correction of replication errors by DNA polymerase using a 3'-5' exonuclease activity.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.89
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

9
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0008408 Catalysis of the hydrolysis of ester linkages within nucleic acids by removing nucleotide residues from the 3' end.
  • GO:0000287 Binding to a magnesium (Mg) ion.
  • GO:0003676 Binding to a nucleic acid.
  • GO:0016896 Catalysis of the hydrolysis of ester linkages within ribonucleic acids by removing nucleotide residues from the 3' or 5' end to yield 5' phosphomonoesters.
  • GO:0045004 Correction of replication errors by DNA polymerase using a 3'-5' exonuclease activity.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
  • GO:0004540 Catalysis of the cleavage of phosphodiester bonds in chains of RNA.
  • GO:0006396 Any process involved in the conversion of one or more primary RNA transcripts into one or more mature RNA molecules.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
1 224 Hamap MF_00157 Ribonuclease T [rnt].
1 224 InterPro IPR005987 Ribonuclease T
32 206 Pfam PF00929 Exonuclease
32 206 InterPro IPR013520 Exonuclease, RNase T/DNA polymerase III
22 221 NCBIfam TIGR01298 ribonuclease T
22 221 InterPro IPR005987 Ribonuclease T
32 216 PANTHER PTHR30231 DNA POLYMERASE III SUBUNIT EPSILON
25 213 CDD cd06134 RNaseT
25 213 InterPro IPR005987 Ribonuclease T
19 224 Gene3D G3DSA:3.30.420.10 -
19 224 InterPro IPR036397 Ribonuclease H superfamily
30 215 SMART SM00479 exoiiiendus
30 215 InterPro IPR013520 Exonuclease, RNase T/DNA polymerase III
21 219 SUPERFAMILY SSF53098 Ribonuclease H-like
21 219 InterPro IPR012337 Ribonuclease H-like superfamily
21 224 FunFam G3DSA:3.30.420.10:FF:000009 Ribonuclease T

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 2F96
X-ray 2.09 Å A,B
100.0% 1-224
PDBe RCSB PDBj File
Viewing
AlphaFold PA3528
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.89
3 0.249

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 8.64 0.462
2 3.05 0.101

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

Show only:
Ligand UniProt (homolog) pchembl MW · LogP · TPSA Lipinski PAINS SMILES
CHEMBL395814 P30014 — 396.4 Da LogP 3.70 TPSA 160.9 ✓ Ro5 ✓ Clean O=C(O)c1cc(SSc2ccc([N+](=O)[O-])c(C(=O)O)c2)ccc…
QU4 P30014 — 422.3 Da LogP 2.45 TPSA 169.4 ✓ Ro5 ✓ Clean c1cc(c(cc1C(=C2C=CC(=O)C(=C2)C(=O)O)c3ccc(c(c3)…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.