Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA3541
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA3541 alg8
Status: annotated
Amino acids: 494
Structure source: AlphaFold

EC

2.4.1.33

GO

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0047643 Catalysis of the reaction: GDP-D-mannuronate + alginate(n) = GDP + alginate(n+1). GO:0050501 Catalysis of the reaction: UDP-D-glucuronate + UDP-N-acetyl-D-glucosamine = [beta-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)](n) + 2n UDP. GO:0042121 The chemical reactions and pathways resulting in the formation of alginic acid, a hydrophilic polysaccharide occurring in, for example, the cell walls of brown algae (brown seaweeds). GO:0085029 The aggregation, arrangement and bonding together of the extracellular matrix. GO:0030213 The chemical reactions and pathways resulting in the formation of hyaluronan, the naturally occurring anionic form of hyaluronic acid. Hyaluronan is a type of non-sulfated glycosaminoglycan composed of the repeating disaccharide unit beta(1,4)-D-glucuronic acid-beta(1,3)-N-acetyl-D-glucosamine.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): N
Localization: CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.64

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

2.4.1.33

Gene Ontology (GO)

7

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0047643 Catalysis of the reaction: GDP-D-mannuronate + alginate(n) = GDP + alginate(n+1).
GO:0050501 Catalysis of the reaction: UDP-D-glucuronate + UDP-N-acetyl-D-glucosamine = [beta-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)](n) + 2n UDP.
GO:0042121 The chemical reactions and pathways resulting in the formation of alginic acid, a hydrophilic polysaccharide occurring in, for example, the cell walls of brown algae (brown seaweeds).
GO:0085029 The aggregation, arrangement and bonding together of the extracellular matrix.
GO:0030213 The chemical reactions and pathways resulting in the formation of hyaluronan, the naturally occurring anionic form of hyaluronic acid. Hyaluronan is a type of non-sulfated glycosaminoglycan composed of the repeating disaccharide unit beta(1,4)-D-glucuronic acid-beta(1,3)-N-acetyl-D-glucosamine.
GO:0000271 The chemical reactions and pathways resulting in the formation of a polysaccharide, a polymer of many (typically more than 10) monosaccharide residues linked glycosidically.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records

Show feature table

Start	End	DB	Term	Name
446	472	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
31	49	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
381	403	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
50	71	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
494	494	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1	28	SignalP_EUK	SignalP-TM	SignalP-TM
1	11	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
473	493	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
359	387	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
417	427	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1	458	PANTHER	PTHR22913	HYALURONAN SYNTHASE
428	445	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
12	30	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
72	358	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
388	392	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
49	71	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
423	445	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
87	343	Pfam	PF13641	Glycosyltransferase like family 2
393	416	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
12	34	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
151	363	SUPERFAMILY	SSF53448	Nucleotide-diphospho-sugar transferases
151	363	InterPro	IPR029044	Nucleotide-diphospho-sugar transferases

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA3541	AlphaFold	—	—	full sequence	—	Viewing

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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
2				0.64

PA3541