Protein profile

PA3546

alginate biosynthesis protein AlgX

Genome: NC_002516.2

Gene: PA3546 algX Structure source: Experimental + AlphaFold UniProt Q51372
Export view
Amino acids 474
Annotations 5
Features 17
PDB binders 0
Druggability 0.373

Overview

Basic information about this protein and its source genome.

Accession
PA3546
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3546 algX
Status
annotated
Amino acids
474
Structure source
Experimental + AlphaFold
EC
2.3.1.-
GO
GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria). GO:0016746 Catalysis of the transfer of an acyl group from one compound (donor) to another (acceptor). GO:0042121 The chemical reactions and pathways resulting in the formation of alginic acid, a hydrophilic polysaccharide occurring in, for example, the cell walls of brown algae (brown seaweeds). GO:0044010 A process in which planktonically growing microorganisms of the same species grow at a liquid-air interface or on a solid substrate under the flow of a liquid and produce extracellular polymers that facilitate matrix formation, resulting in a change in the organisms' growth rate and gene transcription.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Periplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.373
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria).
  • GO:0016746 Catalysis of the transfer of an acyl group from one compound (donor) to another (acceptor).
  • GO:0042121 The chemical reactions and pathways resulting in the formation of alginic acid, a hydrophilic polysaccharide occurring in, for example, the cell walls of brown algae (brown seaweeds).
  • GO:0044010 A process in which planktonically growing microorganisms of the same species grow at a liquid-air interface or on a solid substrate under the flow of a liquid and produce extracellular polymers that facilitate matrix formation, resulting in a change in the organisms' growth rate and gene transcription.

Sequence Features

Domain/signature hits from InterPro and related databases.

17 records
Show feature table
Start End DB Term Name
1 26 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
1 10 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
1 26 Phobius SIGNAL_PEPTIDE Signal peptide region
43 348 CDD cd14441 AlgX_N
43 348 InterPro IPR034655 Alginate biosynthesis protein AlgX, N-terminal
64 325 Pfam PF16822 SGNH hydrolase-like domain, acetyltransferase AlgX
64 325 InterPro IPR031811 AlgX/AlgJ, SGNH hydrolase-like domain
1 26 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
22 26 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
346 463 Gene3D G3DSA:2.60.120.1380 -
346 463 InterPro IPR038639 AlgX, C-terminal domain superfamily
11 21 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
337 461 CDD cd14487 AlgX_C
337 461 InterPro IPR031798 Alginate biosynthesis protein AlgX, C-terminal carbohydrate-binding module
27 474 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
346 467 Pfam PF16824 C-terminal carbohydrate-binding module
346 467 InterPro IPR031798 Alginate biosynthesis protein AlgX, C-terminal carbohydrate-binding module

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 4KNC
X-ray 2.14 Å A,B
94.5% 27-474
PDBe RCSB PDBj File
Viewing
AlphaFold PA3546
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
5 0.373

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 14.84 0.736
2 1.46 0.02
3 1.4 0.017
4 1.18 0.01