Protein profile

PA3560

PTS system fructose-specific transporter subunit IIBC

Genome: NC_002516.2

Gene: PA3560 fruA Structure source: AlphaFold UniProt Q9HY57
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Amino acids 585
Annotations 9
Features 56
PDB binders 0
Druggability 0.837

Overview

Basic information about this protein and its source genome.

Accession
PA3560
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3560 fruA
Status
annotated
Amino acids
585
Structure source
AlphaFold
EC
2.7.1.202
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in). GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0022877 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + fructose(out) = protein histidine + fructose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.837
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in).
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0022877 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + fructose(out) = protein histidine + fructose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

56 records
Show feature table
Start End DB Term Name
402 419 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
522 541 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
463 473 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
239 573 ProSiteProfiles PS51104 PTS_EIIC type-2 domain profile.
239 573 InterPro IPR013014 Phosphotransferase system, EIIC component, type 2
246 508 Pfam PF02378 Phosphotransferase system, EIIC
246 508 InterPro IPR003352 Phosphotransferase system, EIIC
439 462 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
474 495 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 103 Gene3D G3DSA:3.40.50.2300 -
284 306 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 103 FunFam G3DSA:3.40.50.2300:FF:000494 PTS system fructose-specific transporter subunit IIBC
499 521 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
501 521 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
388 410 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
233 564 NCBIfam TIGR01427 fructose-specific PTS transporter subunit EIIC
233 564 InterPro IPR006327 Phosphotransferase system, fructose IIC component
123 206 NCBIfam TIGR00829 fructose PTS transporter subunit IIB
123 206 InterPro IPR003353 Phosphotransferase system, fructose-specific IIB subunit
440 462 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
124 209 Pfam PF02302 PTS system, Lactose/Cellobiose specific IIB subunit
124 209 InterPro IPR003501 Phosphotransferase system, EIIB component, type 2/3
5 94 Pfam PF02302 PTS system, Lactose/Cellobiose specific IIB subunit
5 94 InterPro IPR003501 Phosphotransferase system, EIIB component, type 2/3
564 585 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
496 500 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
319 341 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
361 381 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
116 222 FunFam G3DSA:3.40.50.2300:FF:000014 PTS system fructose-like transporter subunit IIB
243 269 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
2 97 SUPERFAMILY SSF52794 PTS system IIB component-like
2 97 InterPro IPR036095 PTS system IIB component-like superfamily
122 217 CDD cd05569 PTS_IIB_fructose
122 217 InterPro IPR003353 Phosphotransferase system, fructose-specific IIB subunit
541 563 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
117 567 PANTHER PTHR30505 FRUCTOSE-LIKE PERMEASE
247 269 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
289 306 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
122 217 ProSiteProfiles PS51099 PTS_EIIB type-2 domain profile.
122 217 InterPro IPR013011 Phosphotransferase system, EIIB component, type 2
382 401 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
121 218 SUPERFAMILY SSF52794 PTS system IIB component-like
121 218 InterPro IPR036095 PTS system IIB component-like superfamily
1 242 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
542 563 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
342 360 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 98 ProSiteProfiles PS51099 PTS_EIIB type-2 domain profile.
1 98 InterPro IPR013011 Phosphotransferase system, EIIB component, type 2
307 317 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
361 381 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
270 288 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
318 341 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
113 220 Gene3D G3DSA:3.40.50.2300 -
420 438 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
3 98 CDD cd05569 PTS_IIB_fructose
3 98 InterPro IPR003353 Phosphotransferase system, fructose-specific IIB subunit

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3560
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
8 0.808
3 0.7
18 0.593
12 0.269