Protein profile

PA3573

major facilitator superfamily transporter

Genome: NC_002516.2

Gene: PA3573 Structure source: AlphaFold UniProt Q9HY47
Export view
Amino acids 392
Annotations 7
Features 51
PDB binders 4
Druggability 0.946

Overview

Basic information about this protein and its source genome.

Accession
PA3573
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3573
Status
annotated
Amino acids
392
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015385 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: Na+(out) + H+(in) = Na+(in) + H+(out). GO:0042910 Enables the directed movement of a xenobiotic from one side of a membrane to the other. A xenobiotic is a compound foreign to the organism exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical. GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
35.484
Human E-value
5.6e-07
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.946
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015385 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: Na+(out) + H+(in) = Na+(in) + H+(out).
  • GO:0042910 Enables the directed movement of a xenobiotic from one side of a membrane to the other. A xenobiotic is a compound foreign to the organism exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
  • GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.

Sequence Features

Domain/signature hits from InterPro and related databases.

51 records
Show feature table
Start End DB Term Name
61 71 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
157 179 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
273 295 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
208 234 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
246 266 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
361 383 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
244 266 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
43 60 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
180 207 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
5 15 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
300 322 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
327 337 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
6 382 CDD cd17320 MFS_MdfA_MDR_like
152 156 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
303 326 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
3 380 NCBIfam TIGR00710 Bcr/CflA family efflux MFS transporter
3 380 InterPro IPR004812 Drug resistance transporter Bcr/CmlA subfamily
335 357 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
130 152 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
2 389 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
2 389 InterPro IPR020846 Major facilitator superfamily domain
96 117 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
338 357 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
95 117 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
5 383 Gene3D G3DSA:1.20.1720.10 Multidrug resistance protein D
298 302 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
15 348 Pfam PF07690 Major Facilitator Superfamily
15 348 InterPro IPR011701 Major facilitator superfamily
207 229 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
34 42 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
129 151 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
4 381 PANTHER PTHR23502 MAJOR FACILITATOR SUPERFAMILY
235 245 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
358 362 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
267 277 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
157 179 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
3 385 SUPERFAMILY SSF103473 MFS general substrate transporter
3 385 InterPro IPR036259 MFS transporter superfamily
72 90 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
363 382 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
5 383 FunFam G3DSA:1.20.1720.10:FF:000005 Bcr/CflA family efflux transporter
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
278 297 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
69 91 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 33 Phobius SIGNAL_PEPTIDE Signal peptide region
1 4 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
91 95 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
16 33 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
383 392 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
118 128 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
40 62 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3573
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.946
6 0.478

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

70 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
CLM
4ZOW
P0AEY8 323.1 Da LogP 0.91 TPSA 112.7 ✓ Ro5 ✓ Clean c1cc(ccc1[C@H]([C@@H](CO)NC(=O)C(Cl)Cl)O)[N+](=…
DXC
4ZP0
P0AEY8 392.6 Da LogP 4.48 TPSA 77.8 ✓ Ro5 ✓ Clean C[C@H](CCC(=O)O)[C@H]1CC[C@@H]2[C@@]1([C@H](C[C…
KHJ
6OOP
P0AEY8 186.3 Da LogP 1.00 TPSA 7.8 ✓ Ro5 ✓ Clean C[n+]1ccc(cc1)c2cc[n+](cc2)C
LDA
4ZP0
P0AEY8 229.4 Da LogP 4.48 TPSA 23.1 ✓ Ro5 ✓ Clean CCCCCCCCCCCC[N+](C)(C)[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.