Protein profile

PA3581

glycerol uptake facilitator protein

Genome: NC_002516.2

Gene: glpF PA3581 Structure source: AlphaFold UniProt Q51389
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Amino acids 279
Annotations 6
Features 47
PDB binders 2
Druggability 0.835

Overview

Basic information about this protein and its source genome.

Accession
PA3581
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
glpF PA3581
Status
annotated
Amino acids
279
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015254 Enables the energy-independent facilitated diffusion of glycerol through a transmembrane aqueous pore or channel. GO:0015793 The directed movement of glycerol across a membrane. Glycerol is 1,2,3-propanetriol, a sweet, hygroscopic, viscous liquid, widely distributed in nature as a constituent of many lipids. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0015267 Enables the energy-independent facilitated diffusion of a solute through a transmembrane aqueous pore or channel. Stereospecificity is not exhibited but this transport may be specific for a particular molecular species or class of molecules.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
43.29
Human E-value
6.74e-50
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.835
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015254 Enables the energy-independent facilitated diffusion of glycerol through a transmembrane aqueous pore or channel.
  • GO:0015793 The directed movement of glycerol across a membrane. Glycerol is 1,2,3-propanetriol, a sweet, hygroscopic, viscous liquid, widely distributed in nature as a constituent of many lipids.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0015267 Enables the energy-independent facilitated diffusion of a solute through a transmembrane aqueous pore or channel. Stereospecificity is not exhibited but this transport may be specific for a particular molecular species or class of molecules.

Sequence Features

Domain/signature hits from InterPro and related databases.

47 records
Show feature table
Start End DB Term Name
85 90 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
51 75 PRINTS PR00783 Major intrinsic protein family signature
51 75 InterPro IPR000425 Major intrinsic protein
154 172 PRINTS PR00783 Major intrinsic protein family signature
154 172 InterPro IPR000425 Major intrinsic protein
187 209 PRINTS PR00783 Major intrinsic protein family signature
187 209 InterPro IPR000425 Major intrinsic protein
12 31 PRINTS PR00783 Major intrinsic protein family signature
12 31 InterPro IPR000425 Major intrinsic protein
237 257 PRINTS PR00783 Major intrinsic protein family signature
237 257 InterPro IPR000425 Major intrinsic protein
88 107 PRINTS PR00783 Major intrinsic protein family signature
88 107 InterPro IPR000425 Major intrinsic protein
13 257 CDD cd00333 MIP
13 257 InterPro IPR000425 Major intrinsic protein
15 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
236 258 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
69 77 ProSitePatterns PS00221 MIP family signature.
69 77 InterPro IPR022357 Major intrinsic protein, conserved site
10 260 SUPERFAMILY SSF81338 Aquaporin-like
10 260 InterPro IPR023271 Aquaporin-like
89 111 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
67 84 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
16 252 NCBIfam TIGR00861 MIP family channel protein
16 252 InterPro IPR000425 Major intrinsic protein
42 61 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
10 268 PANTHER PTHR43829 AQUAPORIN OR AQUAGLYCEROPORIN RELATED
91 111 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
233 254 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
65 82 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
206 232 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
6 262 Gene3D G3DSA:1.20.1080.10 Glycerol uptake facilitator protein.
6 262 InterPro IPR023271 Aquaporin-like
170 180 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
42 61 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
9 251 Pfam PF00230 Major intrinsic protein
9 251 InterPro IPR000425 Major intrinsic protein
148 169 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
181 205 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
36 41 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
181 203 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 35 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
62 66 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
112 147 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
255 279 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 14 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
147 169 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3581
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.416
4 0.269
5 0.209

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

63 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
3PE
3M9I
Q6J8I9 748.1 Da LogP 12.06 TPSA 134.4 2 viol. ✓ Clean CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(=O)(O)OCCN)OC…
PS6
3D9S
P55064 567.7 Da LogP 5.27 TPSA 171.7 2 viol. ✓ Clean CCCCCCCCCCCCCC(=O)OC[C@@H](CO[P@@](=O)(O)OC[C@H…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.