Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA3642
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA3642 rnhB
Status: annotated
Amino acids: 201
Structure source: AlphaFold

EC

3.1.26.4

GO

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0032299 A protein complex that possesses ribonuclease H activity, in which the catalytic subunit is a member of the RNase H2 (or HII) class. For example, in Saccharomyces the complex contains Rnh201p, Rnh202p and Rnh203p. GO:0030145 Binding to a manganese ion (Mn). GO:0003723 Binding to an RNA molecule or a portion thereof. GO:0004523 Catalysis of the endonucleolytic cleavage of RNA in RNA-DNA hybrids to 5'-phosphomonoesters. GO:0043137 Removal of the Okazaki RNA primer from the lagging strand of replicating DNA, by a combination of the actions of DNA polymerase, DNA helicase and an endonuclease.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.847

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

3.1.26.4

Gene Ontology (GO)

8

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0032299 A protein complex that possesses ribonuclease H activity, in which the catalytic subunit is a member of the RNase H2 (or HII) class. For example, in Saccharomyces the complex contains Rnh201p, Rnh202p and Rnh203p.
GO:0030145 Binding to a manganese ion (Mn).
GO:0003723 Binding to an RNA molecule or a portion thereof.
GO:0004523 Catalysis of the endonucleolytic cleavage of RNA in RNA-DNA hybrids to 5'-phosphomonoesters.
GO:0043137 Removal of the Okazaki RNA primer from the lagging strand of replicating DNA, by a combination of the actions of DNA polymerase, DNA helicase and an endonuclease.
GO:0006298 A system for the correction of errors in which an incorrect base, which cannot form hydrogen bonds with the corresponding base in the parent strand, is incorporated into the daughter strand. The mismatch repair system promotes genomic fidelity by repairing base-base mismatches, insertion-deletion loops and heterologies generated during DNA replication and recombination.
GO:0003676 Binding to a nucleic acid.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records

Show feature table

Start	End	DB	Term	Name
6	199	FunFam	G3DSA:3.30.420.10:FF:000006	Ribonuclease HII
12	201	ProSiteProfiles	PS51975	Ribonuclease (RNase) H type-2 domain profile.
12	201	InterPro	IPR024567	Ribonuclease HII/HIII domain
15	191	Pfam	PF01351	Ribonuclease HII
15	191	InterPro	IPR024567	Ribonuclease HII/HIII domain
13	192	Hamap	MF_00052_B	Ribonuclease HII [rnhB].
13	192	InterPro	IPR022898	Ribonuclease HII
15	191	CDD	cd07182	RNase_HII_bacteria_HII_like
15	191	InterPro	IPR022898	Ribonuclease HII
6	201	Gene3D	G3DSA:3.30.420.10	-
6	201	InterPro	IPR036397	Ribonuclease H superfamily
11	197	PANTHER	PTHR10954	RIBONUCLEASE H2 SUBUNIT A
11	197	InterPro	IPR001352	Ribonuclease HII/HIII
13	197	SUPERFAMILY	SSF53098	Ribonuclease H-like
13	197	InterPro	IPR012337	Ribonuclease H-like superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

Loading 3D structure...

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA3642	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.847
2				0.329

PA3642