Protein profile

PA3805

type 4 fimbrial biogenesis protein PilF

Genome: NC_002516.2

Gene: pilF PA3805 Structure source: Experimental + AlphaFold UniProt Q9HXJ2
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Amino acids 252
Annotations 7
Features 27
PDB binders 0
Druggability 0.242

Overview

Basic information about this protein and its source genome.

Accession
PA3805
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
pilF PA3805
Status
annotated
Amino acids
252
Structure source
Experimental + AlphaFold
GO
GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures. GO:0030911 Binding to a tetratricopeptide repeat (TPR) domain of a protein, the consensus sequence of which is defined by a pattern of small and large hydrophobic amino acids and a structure composed of helices. GO:0009297 The assembly from its constituent parts of a pilus, a short filamentous structure of bacterial cell, flagella-like in structure and generally present in many copies. Pili are variously involved in transfer of nucleic acids, adherence to surfaces, and formation of pellicles. Is required for bacterial conjugation, or can play a role in adherence to surfaces (when it is called a fimbrium), and in the formation of pellicles. GO:0046903 The controlled release of a substance by a cell or a tissue. GO:0043683 The assembly from its constituent parts of a type IV pilus. GO:0043107 Any process involved in the controlled movement of a bacterial cell which is dependent on the presence of type IV pili. Includes social gliding motility and twitching motility.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
28.75
Human E-value
2e-06
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
OuterMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.242
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.
  • GO:0030911 Binding to a tetratricopeptide repeat (TPR) domain of a protein, the consensus sequence of which is defined by a pattern of small and large hydrophobic amino acids and a structure composed of helices.
  • GO:0009297 The assembly from its constituent parts of a pilus, a short filamentous structure of bacterial cell, flagella-like in structure and generally present in many copies. Pili are variously involved in transfer of nucleic acids, adherence to surfaces, and formation of pellicles. Is required for bacterial conjugation, or can play a role in adherence to surfaces (when it is called a fimbrium), and in the formation of pellicles.
  • GO:0046903 The controlled release of a substance by a cell or a tissue.
  • GO:0043683 The assembly from its constituent parts of a type IV pilus.
  • GO:0043107 Any process involved in the controlled movement of a bacterial cell which is dependent on the presence of type IV pili. Includes social gliding motility and twitching motility.
  • GO:0005515 Binding to a protein.

Sequence Features

Domain/signature hits from InterPro and related databases.

27 records
Show feature table
Start End DB Term Name
35 203 PANTHER PTHR44186 -
38 203 SUPERFAMILY SSF48452 TPR-like
38 203 InterPro IPR011990 Tetratricopeptide-like helical domain superfamily
32 252 Gene3D G3DSA:1.25.40.10 Tetratricopeptide repeat domain
32 252 InterPro IPR011990 Tetratricopeptide-like helical domain superfamily
37 69 ProSiteProfiles PS50293 TPR repeat region circular profile.
5 16 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 21 SignalP_EUK SignalP-noTM SignalP-noTM
17 21 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
10 240 NCBIfam TIGR02521 type IV pilus biogenesis/stability protein PilW
10 240 InterPro IPR013360 Pilus biogenesis/stability type IV, PilW
33 251 FunFam G3DSA:1.25.40.10:FF:001817 Type 4 fimbrial biogenesis protein PilF
37 70 ProSiteProfiles PS50005 TPR repeat profile.
37 70 InterPro IPR019734 Tetratricopeptide repeat
1 21 Phobius SIGNAL_PEPTIDE Signal peptide region
22 252 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 4 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
1 22 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
141 174 ProSiteProfiles PS50005 TPR repeat profile.
141 174 InterPro IPR019734 Tetratricopeptide repeat
1 18 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.
105 172 Pfam PF13424 Tetratricopeptide repeat
141 174 SMART SM00028 tpr_5
71 104 SMART SM00028 tpr_5
71 104 InterPro IPR019734 Tetratricopeptide repeat
37 70 SMART SM00028 tpr_5
47 82 Pfam PF13414 TPR repeat

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

2 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 2HO1
X-ray 2.00 Å A,B
96.0% 11-252
PDBe RCSB PDBj File
Viewing
PDB 2FI7
X-ray 2.20 Å A,B
92.5% 20-252
PDBe RCSB PDBj File
Loaded
AlphaFold PA3805
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.242

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.27 0.013