Protein profile

PA3817

methyltransferase

Genome: NC_002516.2

Gene: PA3817 trmJ Structure source: AlphaFold UniProt Q9HXI5
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Amino acids 257
Annotations 8
Features 16
PDB binders 2
Druggability 0.276

Overview

Basic information about this protein and its source genome.

Accession
PA3817
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA3817 trmJ
Status
annotated
Amino acids
257
Structure source
AlphaFold
EC
2.1.1.200
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0003723 Binding to an RNA molecule or a portion thereof. GO:0106339 Catalysis of the reaction: cytidine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32) in tRNA + S-adenosyl-L-homocysteine + H+. GO:0160206 Catalysis of the reaction: cytidine(32)/uridine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methyluridine(32) in tRNA + H+ + S-adenosyl-L-homocysteine. GO:0002128 The process that results in the modification of the sugar of a nucleoside in tRNA at the 2'O position. GO:0008173 Catalysis of the transfer of a methyl group from a donor to a nucleoside residue in an RNA molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.276
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0003723 Binding to an RNA molecule or a portion thereof.
  • GO:0106339 Catalysis of the reaction: cytidine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32) in tRNA + S-adenosyl-L-homocysteine + H+.
  • GO:0160206 Catalysis of the reaction: cytidine(32)/uridine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methyluridine(32) in tRNA + H+ + S-adenosyl-L-homocysteine.
  • GO:0002128 The process that results in the modification of the sugar of a nucleoside in tRNA at the 2'O position.
  • GO:0008173 Catalysis of the transfer of a methyl group from a donor to a nucleoside residue in an RNA molecule.
  • GO:0006396 Any process involved in the conversion of one or more primary RNA transcripts into one or more mature RNA molecules.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
1 177 Gene3D G3DSA:3.40.1280.10 -
1 177 InterPro IPR029026 tRNA (guanine-N1-)-methyltransferase, N-terminal
4 170 SUPERFAMILY SSF75217 alpha/beta knot
4 170 InterPro IPR029028 Alpha/beta knot methyltransferases
5 156 CDD cd18093 SpoU-like_TrmJ
1 245 NCBIfam TIGR00050 TrmJ/YjtD family RNA methyltransferase
1 245 InterPro IPR004384 RNA methyltransferase TrmJ/LasT
5 155 Pfam PF00588 SpoU rRNA Methylase family
5 155 InterPro IPR001537 tRNA/rRNA methyltransferase, SpoU type
178 255 FunFam G3DSA:1.10.8.590:FF:000004 tRNA (cytidine/uridine/adenosine-2'-O-)-methyltransferase TrmJ
178 255 Gene3D G3DSA:1.10.8.590 -
2 247 PANTHER PTHR42786 TRNA/RRNA METHYLTRANSFERASE
2 247 InterPro IPR004384 RNA methyltransferase TrmJ/LasT
1 173 FunFam G3DSA:3.40.1280.10:FF:000006 Uncharacterized tRNA/rRNA methyltransferase HI_0380
1 251 PIRSF PIRSF004808 LasT
1 251 InterPro IPR004384 RNA methyltransferase TrmJ/LasT

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3817
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.276
5 0.253
6 0.232

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
MTA
4CNF
Q4JB16 297.3 Da LogP -0.61 TPSA 119.3 ✓ Ro5 ✓ Clean CSC[C@@H]1[C@H]([C@H]([C@@H](O1)n2cnc3c2ncnc3N)…
SFG
5GM8
A0A072ZPM2 381.4 Da LogP -2.06 TPSA 208.7 2 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.