Protein profile

PA3878

two-component sensor NarX

Genome: NC_002516.2

Gene: GUL26_00735 PA3878 IPC1295_14005 narX PAERUG_P19_London_7_VIM_2_05_10_06654 Structure source: AlphaFold UniProt G3XD66 UniProt O54040
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Amino acids 622
Annotations 9
Features 37
PDB binders 1
Druggability 0.643

Overview

Basic information about this protein and its source genome.

Accession
PA3878
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
GUL26_00735 PA3878 IPC1295_14005 narX PAERUG_P19_London_7_VIM_2_05_10_06654
Status
annotated
Amino acids
622
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0046983 The formation of a protein dimer, a macromolecular structure consists of two noncovalently associated identical or nonidentical subunits. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.643
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MNKATAPDADQRDRFFGGSDRAGKPILRRSLVVRFGCYLTLLVSLALIGMFSALLFADYTHQDAAVINHAGSLRMLTYRVALEPSLEGRQALLGTLGERLDSGDIRRLLQRQDADDPIRQLHQELREGLARLDPRALPGRAVLDAYVGDIDRFVGILQAKAERRSQLLSTVQGLCLFLSLLVVFVTLYDLSYHVIGPLRELTSTARRLGRGELDARVTYSGEDELAQLGERFNQMAGELKSIYGDLEERVEDKTRALSQSHQRLELLYASARRLGENPYDSRTLQPLLNQLEKVLDAGRVTLCLNKDGVERAYSSLTTTERPADFCLQGDCGSCREQASACDQPSSLAQPLLMQPLSIAGHRFGELFIESRSGRLENWQQQLIETFCDNIARTFALSLQQEQESRLALFAERGTIARELHDSLAQSLSYLKIQVSRLGTLLKREAPAEKIEDTLDELREGLNGAYRQLRELLTTFRLSLDEPSLEAALGNAVAEFGERGEVTIELDNRLQHVPLSPNEEIHVLQIVREALSNVVRHSQAQRAWVRLSSQADGQVSIAVEDDGVGFDPQQNRSGHYGLTIMQERGQTLGSQLRFEARAPHGTRVLFSFVPTALQPSAAKEPTP

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0046983 The formation of a protein dimer, a macromolecular structure consists of two noncovalently associated identical or nonidentical subunits.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0004673 Catalysis of the reaction: ATP + protein L-histidine = ADP + protein phospho-L-histidine.

Sequence Features

Domain/signature hits from InterPro and related databases.

37 records
Show feature table
Start End DB Term Name
522 603 CDD cd16917 HATPase_UhpB-NarQ-NarX-like
193 242 SUPERFAMILY SSF158472 HAMP domain-like
17 622 PIRSF PIRSF003167 HK_NarX/NarQ
17 622 InterPro IPR016380 Signal transduction histidine kinase, nitrate/nitrite-sensing
477 610 Gene3D G3DSA:3.30.565.10 -
477 610 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
411 476 Pfam PF07730 Histidine kinase
411 476 InterPro IPR011712 Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain
447 474 Coils Coil Coil
27 613 PANTHER PTHR24421 NITRATE/NITRITE SENSOR PROTEIN NARX-RELATED
189 622 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
391 473 Gene3D G3DSA:1.20.5.1930 -
58 166 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
192 244 SMART SM00304 HAMP_11
192 244 InterPro IPR003660 HAMP domain
62 101 Pfam PF13675 Type IV pili methyl-accepting chemotaxis transducer N-term
62 101 InterPro IPR029095 NarX-like, N-terminal
517 611 SMART SM00387 HKATPase_4
517 611 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
518 608 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
518 608 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
192 240 Pfam PF00672 HAMP domain
192 240 InterPro IPR003660 HAMP domain
414 611 ProSiteProfiles PS50109 Histidine kinase domain profile.
414 611 InterPro IPR005467 Histidine kinase domain
167 188 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
195 239 CDD cd06225 HAMP
31 57 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
161 251 Gene3D G3DSA:6.10.340.10 -
35 57 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 30 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
173 195 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
192 244 ProSiteProfiles PS50885 HAMP domain profile.
508 608 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
508 608 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
63 160 Gene3D G3DSA:1.20.120.960 Histidine kinase NarX, sensor domain
63 160 InterPro IPR042295 NarX-like, N-terminal domain superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA3878
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.643
5 0.631

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

47 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
LFA
5IJI
P27896 282.6 Da LogP 8.05 TPSA 0.0 1 viol. ✓ Clean CCCCCCCCCCCCCCCCCCCC

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.