Protein profile

PA4000

hypothetical protein

Genome: NC_002516.2

Gene: PA4000 rlpA Structure source: Experimental + AlphaFold UniProt Q9X6V6
Export view
Amino acids 342
Annotations 8
Features 30
PDB binders 0
Druggability 0.854

Overview

Basic information about this protein and its source genome.

Accession
PA4000
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4000 rlpA
Status
annotated
Amino acids
342
Structure source
Experimental + AlphaFold
EC
4.2.2.-
GO
GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0008932 Catalysis of the endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue. GO:0042834 Interacting selectively and non-covalently, in a non-covalent manner, with peptidoglycan, any of a class of glycoconjugates found in bacterial cell walls. GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. GO:0000270 The chemical reactions and pathways involving peptidoglycans, any of a class of glycoconjugates found only in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.854
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0008932 Catalysis of the endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
  • GO:0042834 Interacting selectively and non-covalently, in a non-covalent manner, with peptidoglycan, any of a class of glycoconjugates found in bacterial cell walls.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
  • GO:0000270 The chemical reactions and pathways involving peptidoglycans, any of a class of glycoconjugates found only in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.
  • GO:0016829 Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring.

Sequence Features

Domain/signature hits from InterPro and related databases.

30 records
Show feature table
Start End DB Term Name
99 192 FunFam G3DSA:2.40.40.10:FF:000003 Endolytic peptidoglycan transglycosylase RlpA
102 193 NCBIfam TIGR00413 septal ring lytic transglycosylase RlpA family protein
102 193 InterPro IPR012997 Rare lipoprotein A
103 197 SUPERFAMILY SSF50685 Barwin-like endoglucanases
103 197 InterPro IPR036908 RlpA-like domain superfamily
101 189 Pfam PF03330 Lytic transglycolase
101 189 InterPro IPR009009 RlpA-like protein, double-psi beta-barrel domain
9 24 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 20 SignalP_EUK SignalP-TM SignalP-TM
1 27 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.
1 29 Phobius SIGNAL_PEPTIDE Signal peptide region
263 336 Gene3D G3DSA:3.30.70.1070 Sporulation related repeat
263 336 InterPro IPR036680 Sporulation-like domain superfamily
1 8 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
25 29 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 30 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
265 334 Pfam PF05036 SPOR domain
265 334 InterPro IPR007730 Sporulation-like domain
265 337 SUPERFAMILY SSF110997 Sporulation related repeat
265 337 InterPro IPR036680 Sporulation-like domain superfamily
30 342 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 31 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
261 342 ProSiteProfiles PS51724 SPOR domain profile.
261 342 InterPro IPR007730 Sporulation-like domain
7 192 Hamap MF_02071 Endolytic peptidoglycan transglycosylase RlpA [rlpA].
7 192 InterPro IPR034718 Endolytic peptidoglycan transglycosylase RlpA
100 193 Gene3D G3DSA:2.40.40.10 -
100 193 InterPro IPR036908 RlpA-like domain superfamily
100 190 CDD cd22268 DPBB_RlpA-like
7 333 PANTHER PTHR34183 -

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

4 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 6I05
X-ray 1.21 Å A
23.1% 264-342
PDBe RCSB PDBj File
Viewing
PDB 6I09
X-ray 1.48 Å A
22.8% 265-342
PDBe RCSB PDBj File
Loaded
PDB 6I0A
X-ray 1.30 Å A
22.5% 266-342
PDBe RCSB PDBj File
Loaded
PDB 6I0N
X-ray 1.40 Å A
22.5% 266-342
PDBe RCSB PDBj File
Loaded
AlphaFold PA4000
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.854