Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA4001
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: sltB1 PA4001
Status: annotated
Amino acids: 340
Structure source: Experimental + AlphaFold

GO

GO:0046872 Binding to a metal ion. GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand. GO:0009253 The chemical reactions and pathways resulting in the breakdown of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): N
Localization: CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.804

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3

GO:0046872 Binding to a metal ion.
GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand.
GO:0009253 The chemical reactions and pathways resulting in the breakdown of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.

Sequence Features

Domain/signature hits from InterPro and related databases.

17 records

Show feature table

Start	End	DB	Term	Name
135	215	FunFam	G3DSA:1.10.8.350:FF:000001	Lytic murein transglycosylase B
44	332	NCBIfam	TIGR02282	lytic murein transglycosylase B
44	332	InterPro	IPR011757	Lytic transglycosylase MltB
1	15	Phobius	SIGNAL_PEPTIDE_N_REGION	N-terminal region of a signal peptide.
47	215	Gene3D	G3DSA:1.10.8.350	Bacterial muramidase
29	339	PANTHER	PTHR30163	MEMBRANE-BOUND LYTIC MUREIN TRANSGLYCOSYLASE B
29	339	InterPro	IPR043426	Membrane-bound lytic murein transglycosylase B-like
28	33	Phobius	SIGNAL_PEPTIDE_C_REGION	C-terminal region of a signal peptide.
40	330	Pfam	PF13406	Transglycosylase SLT domain
40	330	InterPro	IPR031304	Transglycosylase SLT domain 2
81	330	Gene3D	G3DSA:1.10.530.10	-
1	33	Phobius	SIGNAL_PEPTIDE	Signal peptide region
30	334	SUPERFAMILY	SSF53955	Lysozyme-like
30	334	InterPro	IPR023346	Lysozyme-like domain superfamily
34	340	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
16	27	Phobius	SIGNAL_PEPTIDE_H_REGION	Hydrophobic region of a signal peptide.
121	330	CDD	cd13399	Slt35-like

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

Loading 3D structure...

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Structural evidence

2 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
PDB 5O8X	X-ray	2.50 Å	A,B	90.0% 35-340	PDBe RCSB PDBj File	Viewing
PDB 4ANR	X-ray	1.84 Å	A	89.1% 38-340	PDBe RCSB PDBj File	Loaded
AlphaFold PA4001	AlphaFold	—	—	full sequence	—	Loaded

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.459

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

2 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

No PDB structure with a co-crystallized ligand found for this exact protein.

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Ligand	Source crystal	UniProt (homolog)	MW · LogP · TPSA	Lipinski	PAINS	SMILES
BLG	1D0L	P41052	552.6 Da LogP -6.16 TPSA 271.9	3 viol.	✓ Clean	`CC(=O)N[C@@H]1[C@H]([C@@H]([C@H](O[C@H]1O[C@H]2…`

Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL bioactivity data found for this exact protein.

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL hits found through similar proteins.

Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).

Ligand	Tanimoto	MW · LogP · TPSA	Lipinski	PAINS	SMILES
ZINC34074657	0.562	444.4 Da LogP -4.05 TPSA 221.2	2 viol.	✓ Clean	`CC(=O)N[C@H]1[C@H](O[C@H]2C[C@@H](C(=O)O)N[C@@H…`

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.

PA4001