Protein profile

PA4036

two-component sensor

Genome: NC_002516.2

Gene: PA4036 Structure source: AlphaFold UniProt Q9HWZ1
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Amino acids 766
Annotations 9
Features 52
PDB binders 1
Druggability 0.916

Overview

Basic information about this protein and its source genome.

Accession
PA4036
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4036
Status
annotated
Amino acids
766
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0009927 Serves as a phospho-His intermediate enabling the transfer of phospho group between a hybrid kinase and a response regulator. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.916
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0009927 Serves as a phospho-His intermediate enabling the transfer of phospho group between a hybrid kinase and a response regulator.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

52 records
Show feature table
Start End DB Term Name
603 762 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
603 762 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
552 763 ProSiteProfiles PS50109 Histidine kinase domain profile.
552 763 InterPro IPR005467 Histidine kinase domain
433 500 SMART SM00091 pas_2
433 500 InterPro IPR000014 PAS domain
35 322 Pfam PF05226 CHASE2 domain
35 322 InterPro IPR007890 CHASE2
543 604 CDD cd00082 HisKA
543 604 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
357 374 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
25 38 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
418 535 SUPERFAMILY SSF55785 PYP-like sensor domain (PAS domain)
418 535 InterPro IPR035965 PAS domain superfamily
419 541 Gene3D G3DSA:3.30.450.20 PAS domain
329 332 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
542 611 Gene3D G3DSA:1.10.287.130 -
352 356 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
375 766 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
340 761 PANTHER PTHR43047 TWO-COMPONENT HISTIDINE PROTEIN KINASE
306 328 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
333 351 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
309 328 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
356 375 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
653 763 SMART SM00387 HKATPase_4
653 763 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
654 761 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
654 761 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
43 326 SMART SM01080 CHASE2_2
43 326 InterPro IPR007890 CHASE2
537 608 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
537 608 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
690 704 PRINTS PR00344 Bacterial sensor protein C-terminal signature
690 704 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
723 741 PRINTS PR00344 Bacterial sensor protein C-terminal signature
723 741 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
747 760 PRINTS PR00344 Bacterial sensor protein C-terminal signature
747 760 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
2 766 PIRSF PIRSF037347 HK_CHASE2_PAS
2 766 InterPro IPR017181 Signal transduction histidine kinase, CHASE2 sensor domain-containing, predicted
1 43 Phobius SIGNAL_PEPTIDE Signal peptide region
439 540 Pfam PF08448 PAS fold
439 540 InterPro IPR013656 PAS fold-4
44 308 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
333 352 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
618 765 FunFam G3DSA:3.30.565.10:FF:000085 Sensor histidine kinase KdpD
39 43 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 24 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
613 764 Gene3D G3DSA:3.30.565.10 -
613 764 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
24 46 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
658 759 CDD cd00075 HATPase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4036
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
16 0.707
24 0.542
2 0.485

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

51 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.