Protein profile

PA4232

single-stranded DNA-binding protein

Genome: NC_002516.2

Gene: ssb PA4232 Structure source: Experimental + AlphaFold UniProt P40947
Export view
Amino acids 165
Annotations 6
Features 21
PDB binders 2
Druggability 0.245

Overview

Basic information about this protein and its source genome.

Accession
PA4232
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
ssb PA4232
Status
annotated
Amino acids
165
Structure source
Experimental + AlphaFold
GO
GO:0009295 The region of a virus, bacterial cell, mitochondrion or chloroplast to which the nucleic acid is confined. GO:0008047 A molecular function regulator that increases a catalytic activity. GO:0003697 Binding to single-stranded DNA. GO:0006310 Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction. GO:0006281 The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway. GO:0006260 The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by the origin recognition complex, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
39.326
Human E-value
2.7e-14
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.245
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0009295 The region of a virus, bacterial cell, mitochondrion or chloroplast to which the nucleic acid is confined.
  • GO:0008047 A molecular function regulator that increases a catalytic activity.
  • GO:0003697 Binding to single-stranded DNA.
  • GO:0006310 Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction.
  • GO:0006281 The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
  • GO:0006260 The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by the origin recognition complex, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
1 159 PIRSF PIRSF002070 SSB
1 159 InterPro IPR011344 Single-stranded DNA-binding protein
5 110 ProSiteProfiles PS50935 Single-strand binding (SSB) domain profile.
5 110 InterPro IPR000424 Primosome PriB/single-strand DNA-binding
5 111 Pfam PF00436 Single-strand binding protein family
5 111 InterPro IPR000424 Primosome PriB/single-strand DNA-binding
5 116 Hamap MF_00984 Single-stranded DNA-binding protein.
5 116 InterPro IPR011344 Single-stranded DNA-binding protein
1 154 FunFam G3DSA:2.40.50.140:FF:000065 Single-stranded DNA-binding protein
3 165 NCBIfam TIGR00621 single-stranded DNA-binding protein
3 165 InterPro IPR011344 Single-stranded DNA-binding protein
3 121 PANTHER PTHR10302 SINGLE-STRANDED DNA-BINDING PROTEIN
3 121 InterPro IPR011344 Single-stranded DNA-binding protein
110 165 MobiDBLite mobidb-lite consensus disorder prediction
127 142 MobiDBLite mobidb-lite consensus disorder prediction
8 104 CDD cd04496 SSB_OBF
8 104 InterPro IPR000424 Primosome PriB/single-strand DNA-binding
3 165 SUPERFAMILY SSF50249 Nucleic acid-binding proteins
3 165 InterPro IPR012340 Nucleic acid-binding, OB-fold
1 155 Gene3D G3DSA:2.40.50.140 -
1 155 InterPro IPR012340 Nucleic acid-binding, OB-fold

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

5 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 6IRQ
X-ray 1.91 Å A,B,C,D
69.7% 1-115
PDBe RCSB PDBj File
Viewing
PDB 5YUO
X-ray 2.04 Å A,B,C,D
69.7% 1-115
PDBe RCSB PDBj File
Loaded
PDB 6JDG
X-ray 2.39 Å A,B,C,D
69.7% 1-115
PDBe RCSB PDBj File
Loaded
PDB 5YUN
X-ray 2.67 Å A,B,C,D
69.7% 1-115
PDBe RCSB PDBj File
Loaded
PDB 7VUM
X-ray 2.32 Å A,B,C,D
69.7% 1-115
PDBe RCSB PDBj File
Metadata only
AlphaFold PA4232
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.245

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Show only:
Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
MYC
5YUN 2.67 Å Open crystal
318.2 Da LogP 1.69 TPSA 151.6 1 viol. Alert c1c(cc(c(c1O)O)O)C2=C(C(=O)c3c(cc(cc3O2)O)O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.