Protein profile

PA4309

chemotactic transducer PctA

Genome: NC_002516.2

Gene: PA4309 pctA Structure source: Experimental + AlphaFold UniProt G3XD24
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Amino acids 629
Annotations 7
Features 45
PDB binders 2
Druggability 0.834

Overview

Basic information about this protein and its source genome.

Accession
PA4309
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4309 pctA
Status
annotated
Amino acids
629
Structure source
Experimental + AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016597 Binding to an amino acid, organic acids containing one or more amino substituents. GO:0004888 Combining with an extracellular or intracellular signal and transmitting the signal from one side of the membrane to the other to initiate a change in cell activity or state as part of signal transduction. GO:0006935 The directed movement of a motile cell or organism, or the directed growth of a cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis). GO:0043200 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an amino acid stimulus. An amino acid is a carboxylic acids containing one or more amino groups. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
25.676
Human E-value
9.48e-09
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.834
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016597 Binding to an amino acid, organic acids containing one or more amino substituents.
  • GO:0004888 Combining with an extracellular or intracellular signal and transmitting the signal from one side of the membrane to the other to initiate a change in cell activity or state as part of signal transduction.
  • GO:0006935 The directed movement of a motile cell or organism, or the directed growth of a cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis).
  • GO:0043200 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an amino acid stimulus. An amino acid is a carboxylic acids containing one or more amino groups.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Sequence Features

Domain/signature hits from InterPro and related databases.

45 records
Show feature table
Start End DB Term Name
404 424 MobiDBLite mobidb-lite consensus disorder prediction
54 273 Gene3D G3DSA:3.30.450.20 PAS domain
66 174 Gene3D G3DSA:3.30.450.20 PAS domain
357 593 ProSiteProfiles PS50111 Bacterial chemotaxis sensory transducers domain profile.
357 593 InterPro IPR004089 Methyl-accepting chemotaxis protein (MCP) signalling domain
302 629 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
435 455 Coils Coil Coil
301 347 CDD cd06225 HAMP
279 301 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
544 606 ProSiteProfiles PS50192 t-SNARE coiled-coil homology domain profile.
544 606 InterPro IPR000727 Target SNARE coiled-coil homology domain
11 628 PANTHER PTHR32089 METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB
172 272 FunFam G3DSA:3.30.450.20:FF:000132 Methyl-accepting chemotaxis protein PctA
394 593 CDD cd11386 MCP_signal
66 171 FunFam G3DSA:3.30.450.20:FF:000048 Methyl-accepting chemotaxis protein
60 176 CDD cd12913 PDC1_MCP_like
411 594 Pfam PF00015 Methyl-accepting chemotaxis protein (MCP) signalling domain
411 594 InterPro IPR004089 Methyl-accepting chemotaxis protein (MCP) signalling domain
298 629 FunFam G3DSA:1.10.287.950:FF:000001 Methyl-accepting chemotaxis sensory transducer
30 277 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
367 628 SMART SM00283 MA_2
367 628 InterPro IPR004089 Methyl-accepting chemotaxis protein (MCP) signalling domain
36 259 Pfam PF02743 Cache domain
36 259 InterPro IPR033479 Double Cache domain 1
320 629 SUPERFAMILY SSF58104 Methyl-accepting chemotaxis protein (MCP) signaling domain
298 352 ProSiteProfiles PS50885 HAMP domain profile.
298 352 InterPro IPR003660 HAMP domain
91 184 SUPERFAMILY SSF103190 Sensory domain-like
91 184 InterPro IPR029151 Periplasmic sensor-like domain superfamily
10 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 29 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
178 265 CDD cd12912 PDC2_MCP_like
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
298 352 SMART SM00304 HAMP_11
387 447 SMART SM00304 HAMP_11
387 447 InterPro IPR003660 HAMP domain
295 349 Pfam PF00672 HAMP domain
295 349 InterPro IPR003660 HAMP domain
494 523 PRINTS PR00260 Bacterial chemotaxis sensory transducer signature
494 523 InterPro IPR004090 Chemotaxis methyl-accepting receptor
381 410 PRINTS PR00260 Bacterial chemotaxis sensory transducer signature
381 410 InterPro IPR004090 Chemotaxis methyl-accepting receptor
299 629 Gene3D G3DSA:1.10.287.950 -
278 301 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
498 521 Coils Coil Coil

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

3 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 5LTX
X-ray 2.02 Å A,B
39.6% 30-278
PDBe RCSB PDBj File
Viewing
PDB 5T65
X-ray 2.20 Å A,B
39.6% 30-278
PDBe RCSB PDBj File
Loaded
PDB 5T7M
X-ray 2.25 Å A,B
39.6% 30-278
PDBe RCSB PDBj File
Loaded
AlphaFold PA4309
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.834
1 0.75

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 8.6 0.46
2 3.23 0.112

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
HSM
6FU4
Q9I0I4 111.1 Da LogP -0.09 TPSA 54.7 ✓ Ro5 ✓ Clean c1c(nc[nH]1)CCN
TAU
5AVF
Q9KL26 125.1 Da LogP -1.17 TPSA 80.4 ✓ Ro5 ✓ Clean C(CS(=O)(=O)O)N

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.