Protein target profile

PA4343

major facilitator superfamily transporter

Genome: NC_002516.2

Gene: PA4343 3D evidence: AlphaFold DB model UniProt Q9HW58
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Length 439
Pocket druggability 0.711
EC / GO 0 / 5
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

4 signals

Strengths

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Strong predicted binding pocket FPocket selected-pocket score 0.71. This is a computational pocket signal, not binding validation. Structure available AlphaFold DB model

Risks / watch

No major risk signal surfaced in the loaded evidence.

Missing evidence

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
How to read this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal TPW pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Overview

Basic information about this protein and its source genome.

Accession
PA4343
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4343
Status
annotated
Amino acids
439
3D evidence
AlphaFold DB model
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015293 Enables the active transport of a solute across a membrane by a mechanism whereby two or more species are transported together in the same direction in a tightly coupled process not directly linked to a form of energy other than chemiosmotic energy. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
Hit
Essential (DEG)
Y
Localization
CytoplasmicMembrane

Selected pocket evidence

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.711
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MHACPSGTSRTRQVAAAVIGNALEWYDFIVYGFLSSLIARLFFPSGDEYTSLLMALATFGVGFFMRPVGGVLLGLYADRRGRKAAMQLIILLMTLSIAMIAFAPTYAAIGVGAPLLIVIARMLQGFATGGEYASATAFLVESAPPHRRGLYGSWQLFGQCLAVFAGAGMGALVTHCLDAEALESWGWRVPFLFGLLIGPVGLWIRRYMGETEAFLEAIREPGERQGLLGVLREYRRSVLVSMGLTVVGTVSFYVVLVNMPTFAHKQLGLPLDEVFMVQMAAVALMTLVIPLAGGLSDRVGRRPVLLVATLAFMLMVYPLFAWVAAAPSLGRLLLMQLLLCTAIGGFFGPAPTAVAEQFPVRVRSTGLAVAYNLAVMLFGGFAPFIVTWLTEVGGSPVAPAFYVLGAAFLGLLATLYLREGATPAPRPREPALGKPARSL

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015293 Enables the active transport of a solute across a membrane by a mechanism whereby two or more species are transported together in the same direction in a tightly coupled process not directly linked to a form of energy other than chemiosmotic energy.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Sequence Features

Domain/signature hits from InterPro and related databases.

56 records
Show feature table
Start End DB Term Name
12 234 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
12 234 InterPro IPR036259 MFS transporter superfamily
77 87 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
256 274 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
141 151 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
332 355 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 24 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
185 204 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
152 173 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
332 354 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
10 227 FunFam G3DSA:1.20.1250.20:FF:000001 Dicarboxylate MFS transporter
115 140 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
327 331 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
9 424 PANTHER PTHR43528 ALPHA-KETOGLUTARATE PERMEASE
16 211 Pfam PF00083 Sugar (and other) transporter
16 211 InterPro IPR005828 Major facilitator, sugar transporter-like
232 408 Pfam PF00083 Sugar (and other) transporter
232 408 InterPro IPR005828 Major facilitator, sugar transporter-like
237 255 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
11 419 SUPERFAMILY SSF103473 MFS general substrate transporter
11 419 InterPro IPR036259 MFS transporter superfamily
304 326 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
275 292 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
14 421 CDD cd17368 MFS_CitA
398 417 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
88 109 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
274 296 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 422 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
13 422 InterPro IPR020846 Major facilitator superfamily domain
293 303 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
44 48 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
88 110 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
419 439 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
400 418 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
205 236 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
303 322 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
367 388 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
25 43 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
389 399 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
174 184 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
237 259 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
123 148 ProSitePatterns PS00217 Sugar transport proteins signature 2.
123 148 InterPro IPR005829 Sugar transporter, conserved site
235 439 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
235 439 InterPro IPR036259 MFS transporter superfamily
150 172 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
110 114 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
356 366 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
53 75 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
115 137 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
292 309 ProSitePatterns PS00216 Sugar transport proteins signature 1.
292 309 InterPro IPR005829 Sugar transporter, conserved site
366 388 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
49 76 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
187 204 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

3D visualization script Full viewer

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Structural evidence

0 + 1

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB PA4343
AlphaFold DB — — full sequence — Viewing
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Site 1 FPocket #1
0.419
Unusual size
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Site 2 FPocket #6
0.415
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