Protein profile

PA4380

two-component sensor

Genome: NC_002516.2

Gene: PA4380 Structure source: AlphaFold UniProt Q9HW22
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Amino acids 426
Annotations 8
Features 39
PDB binders 2
Druggability 0.646

Overview

Basic information about this protein and its source genome.

Accession
PA4380
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4380
Status
annotated
Amino acids
426
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.646
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MEYKQSLARRIVIAFMLMTVAVGGLFSAGIVGVVHIIEERLISRDLGGELERILRDDLAQGRNPVLDPGMRFFISDGQGSYAMPPALDQLDVGFHEVFEGDLSFHALVRDIDGRRFVLLQDQSDFEAREQVLYASVLTGYVLSIALAGLLGWMLARKVMEPVVRLARQVRHREQLLGLAPPLAPDYANDEVGELAASFDETLGRLRDALKREQLFTSDVSHELRTPLMVIATSCELLAEEPSLGPRARGQLERMTKATEEMRDLVQTFLLLARAQKGEESLAPHGSLESIADDLVQVWREQVEARGLTLHYRNEGACAGQFNAPLLRAVMGNLLRNATHYTDAGSITLTLDEHGFSVEDTGAGVPEEQRERIFMPFVRGSSSSGRGEGLGLGLSLVKRICAAEGWSVTLSAVEPHGCRFEVLLDVA

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Sequence Features

Domain/signature hits from InterPro and related databases.

39 records
Show feature table
Start End DB Term Name
156 426 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
120 208 Gene3D G3DSA:6.10.340.10 -
156 210 ProSiteProfiles PS50885 HAMP domain profile.
156 210 InterPro IPR003660 HAMP domain
132 154 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
325 424 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
325 424 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
15 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
211 277 SMART SM00388 HisKA_10
211 277 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
214 276 Pfam PF00512 His Kinase A (phospho-acceptor) domain
214 276 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
287 423 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
287 423 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
353 367 PRINTS PR00344 Bacterial sensor protein C-terminal signature
353 367 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
371 381 PRINTS PR00344 Bacterial sensor protein C-terminal signature
371 381 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
387 405 PRINTS PR00344 Bacterial sensor protein C-terminal signature
387 405 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
156 210 SMART SM00304 HAMP_11
194 277 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
194 277 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
12 37 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
209 279 Gene3D G3DSA:1.10.287.130 -
209 273 CDD cd00082 HisKA
209 273 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
154 207 Pfam PF00672 HAMP domain
154 207 InterPro IPR003660 HAMP domain
131 155 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
321 426 SMART SM00387 HKATPase_4
321 426 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
218 426 ProSiteProfiles PS50109 Histidine kinase domain profile.
218 426 InterPro IPR005467 Histidine kinase domain
284 426 Gene3D G3DSA:3.30.565.10 -
284 426 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
4 423 PANTHER PTHR45436 SENSOR HISTIDINE KINASE YKOH
38 130 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4380
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.646
5 0.206

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.