Protein profile

PA4420

S-adenosyl-methyltransferase MraW

Genome: NC_002516.2

Gene: mraW PA4420 rsmH Structure source: AlphaFold UniProt Q9HVZ5
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Amino acids 313
Annotations 5
Features 19
PDB binders 0
Druggability 0.61

Overview

Basic information about this protein and its source genome.

Accession
PA4420
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
mraW PA4420 rsmH
Status
annotated
Amino acids
313
Structure source
AlphaFold
EC
2.1.1.199
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0071424 Catalysis of the reaction: a cytidine in rRNA + S-adenosyl-L-methionine = an N(4)-methylcytidine in rRNA + H+ + S-adenosyl-L-homocysteine. GO:0070475 The addition of a methyl group to an atom in the nucleoside base portion of a nucleotide residue in an rRNA molecule. GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
41.799
Human E-value
4.83e-36
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.61
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0071424 Catalysis of the reaction: a cytidine in rRNA + S-adenosyl-L-methionine = an N(4)-methylcytidine in rRNA + H+ + S-adenosyl-L-homocysteine.
  • GO:0070475 The addition of a methyl group to an atom in the nucleoside base portion of a nucleotide residue in an rRNA molecule.
  • GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
114 216 Gene3D G3DSA:1.10.150.170 Putative methyltransferase TM0872, insert domain
114 216 InterPro IPR023397 S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily
4 311 NCBIfam TIGR00006 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH
4 311 InterPro IPR002903 Ribosomal RNA small subunit methyltransferase H
116 215 SUPERFAMILY SSF81799 Putative methyltransferase TM0872, insert domain
116 215 InterPro IPR023397 S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily
1 313 PIRSF PIRSF004486 MraW
1 313 InterPro IPR002903 Ribosomal RNA small subunit methyltransferase H
10 311 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
10 311 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
5 311 Pfam PF01795 MraW methylase family
5 311 InterPro IPR002903 Ribosomal RNA small subunit methyltransferase H
4 312 PANTHER PTHR11265 S-ADENOSYL-METHYLTRANSFERASE MRAW
4 312 InterPro IPR002903 Ribosomal RNA small subunit methyltransferase H
114 216 FunFam G3DSA:1.10.150.170:FF:000003 Ribosomal RNA small subunit methyltransferase H
7 312 SUPERFAMILY SSF53335 S-adenosyl-L-methionine-dependent methyltransferases
7 312 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
7 312 Hamap MF_01007 Ribosomal RNA small subunit methyltransferase H [rsmH].
7 312 InterPro IPR002903 Ribosomal RNA small subunit methyltransferase H

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4420
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.61
4 0.203