Protein profile

PA4482

aspartyl/glutamyl-tRNA amidotransferase subunit C

Genome: NC_002516.2

Gene: PA4482 gatC Structure source: Experimental + AlphaFold UniProt Q9HVT9
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Amino acids 96
Annotations 7
Features 12
PDB binders 0
Druggability 0.508

Overview

Basic information about this protein and its source genome.

Accession
PA4482
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4482 gatC
Status
annotated
Amino acids
96
Structure source
Experimental + AlphaFold
EC
6.3.5.-
GO
GO:0050566 Catalysis of the reaction: L-glutamine + aspartyl-tRNA(Asn) + ATP = L-glutamate + asparaginyl-tRNA(Asn) + phosphate + ADP. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0050567 Catalysis of the reaction: L-glutamine + glutamyl-tRNA(Gln) + ATP = L-glutamate + glutaminyl-tRNA(Gln) + phosphate + ADP. GO:0070681 A tRNA aminoacylation process in which glutaminyl-tRNAGln is formed by a tRNA-dependent two-step pathway. In the first step a non-discriminating glutamyl-tRNAGlx synthetase generates the misacylated L-glutamyl-tRNAGln species, and in the second step it is amidated to the correctly charged L-glutaminyl-tRNAGln by a glutamyl-tRNAGln amidotransferase. GO:0006450 Any process that modulates the ability of the translational apparatus to interpret the genetic code. GO:0006412 The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.508
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0050566 Catalysis of the reaction: L-glutamine + aspartyl-tRNA(Asn) + ATP = L-glutamate + asparaginyl-tRNA(Asn) + phosphate + ADP.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0050567 Catalysis of the reaction: L-glutamine + glutamyl-tRNA(Gln) + ATP = L-glutamate + glutaminyl-tRNA(Gln) + phosphate + ADP.
  • GO:0070681 A tRNA aminoacylation process in which glutaminyl-tRNAGln is formed by a tRNA-dependent two-step pathway. In the first step a non-discriminating glutamyl-tRNAGlx synthetase generates the misacylated L-glutamyl-tRNAGln species, and in the second step it is amidated to the correctly charged L-glutaminyl-tRNAGln by a glutamyl-tRNAGln amidotransferase.
  • GO:0006450 Any process that modulates the ability of the translational apparatus to interpret the genetic code.
  • GO:0006412 The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome.

Sequence Features

Domain/signature hits from InterPro and related databases.

12 records
Show feature table
Start End DB Term Name
2 95 Hamap MF_00122 Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C [gatC].
2 95 InterPro IPR003837 Glu-tRNAGln amidotransferase C subunit
3 93 SUPERFAMILY SSF141000 Glu-tRNAGln amidotransferase C subunit
3 93 InterPro IPR036113 Glu-tRNAGln amidotransferase superfamily, subunit C
3 94 PANTHER PTHR15004 UNCHARACTERIZED
3 94 InterPro IPR003837 Glu-tRNAGln amidotransferase C subunit
4 95 NCBIfam TIGR00135 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC
4 95 InterPro IPR003837 Glu-tRNAGln amidotransferase C subunit
94 96 Coils Coil Coil
19 90 Pfam PF02686 Glu-tRNAGln amidotransferase C subunit
19 90 InterPro IPR003837 Glu-tRNAGln amidotransferase C subunit
1 45 Gene3D G3DSA:1.10.20.60 -

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 4WJ3
X-ray 3.70 Å C,F,I,L
100.0% 1-96
PDBe RCSB PDBj File
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AlphaFold PA4482
AlphaFold — — full sequence — Loaded
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Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 15.04 0.743
2 2.29 0.058
3 1.72 0.03
4 1.72 0.03
5 0.94 0.005