Protein profile

PA4485

hypothetical protein

Genome: NC_002516.2

Gene: rlpA PA4485 Structure source: Experimental + AlphaFold UniProt Q9HVT6
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Amino acids 125
Annotations 5
Features 20
PDB binders 0
Druggability 0.407

Overview

Basic information about this protein and its source genome.

Accession
PA4485
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
rlpA PA4485
Status
annotated
Amino acids
125
Structure source
Experimental + AlphaFold
EC
4.2.2.-
GO
GO:0008932 Catalysis of the endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue. GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. GO:0000270 The chemical reactions and pathways involving peptidoglycans, any of a class of glycoconjugates found only in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides. GO:0016829 Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.407
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0008932 Catalysis of the endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
  • GO:0000270 The chemical reactions and pathways involving peptidoglycans, any of a class of glycoconjugates found only in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.
  • GO:0016829 Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring.

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records
Show feature table
Start End DB Term Name
23 31 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
33 122 CDD cd22268 DPBB_RlpA-like
32 125 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
30 121 SUPERFAMILY SSF50685 Barwin-like endoglucanases
30 121 InterPro IPR036908 RlpA-like domain superfamily
1 31 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
31 125 Gene3D G3DSA:2.40.40.10 -
31 125 InterPro IPR036908 RlpA-like domain superfamily
33 121 Pfam PF03330 Lytic transglycolase
33 121 InterPro IPR009009 RlpA-like protein, double-psi beta-barrel domain
1 31 Phobius SIGNAL_PEPTIDE Signal peptide region
5 124 Hamap MF_02071 Endolytic peptidoglycan transglycosylase RlpA [rlpA].
5 124 InterPro IPR034718 Endolytic peptidoglycan transglycosylase RlpA
1 31 SignalP_EUK SignalP-noTM SignalP-noTM
34 124 NCBIfam TIGR00413 septal ring lytic transglycosylase RlpA family protein
34 124 InterPro IPR012997 Rare lipoprotein A
28 125 PANTHER PTHR34183 -
1 9 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
10 22 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 26 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 4AVR
X-ray 1.08 Å A,B
75.2% 32-125
PDBe RCSB PDBj File
Viewing
AlphaFold PA4485
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.407

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 6.54 0.331