Protein profile

PA4515

hydroxylase

Genome: NC_002516.2

Gene: PA4515 piuC Structure source: Experimental + AlphaFold UniProt Q9HVQ7
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Amino acids 226
Annotations 8
Features 16
PDB binders 0
Druggability 0.619

Overview

Basic information about this protein and its source genome.

Accession
PA4515
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4515 piuC
Status
annotated
Amino acids
226
Structure source
Experimental + AlphaFold
EC
1.14.11.-
GO
GO:0016706 Catalysis of the reaction: A + 2-oxoglutarate + O2 = B + succinate + CO2. This is an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from 2-oxoglutarate and one other donor, and one atom of oxygen is incorporated into each donor. GO:0005506 Binding to an iron (Fe) ion. GO:0031418 Binding to L-ascorbic acid, (2R)-2-[(1S)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2,5-dihydrofuran-3-olate; L-ascorbic acid is vitamin C and has co-factor and anti-oxidant activities in many species. GO:0071281 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an iron ion stimulus. GO:0006974 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism. GO:0006879 A homeostatic process involved in the maintenance of a steady state level of iron ions within a cell.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.619
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0016706 Catalysis of the reaction: A + 2-oxoglutarate + O2 = B + succinate + CO2. This is an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from 2-oxoglutarate and one other donor, and one atom of oxygen is incorporated into each donor.
  • GO:0005506 Binding to an iron (Fe) ion.
  • GO:0031418 Binding to L-ascorbic acid, (2R)-2-[(1S)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2,5-dihydrofuran-3-olate; L-ascorbic acid is vitamin C and has co-factor and anti-oxidant activities in many species.
  • GO:0071281 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an iron ion stimulus.
  • GO:0006974 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
  • GO:0006879 A homeostatic process involved in the maintenance of a steady state level of iron ions within a cell.
  • GO:0016705 Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from each of two donors, and molecular oxygen is reduced or incorporated into a donor.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
1 226 Hamap MF_00657 PKHD-type hydroxylase YbiX [ybiX].
1 226 InterPro IPR023550 PKHD-type hydroxylase
1 177 SMART SM00702 p4hc
1 177 InterPro IPR006620 Prolyl 4-hydroxylase, alpha subunit
1 226 PANTHER PTHR41536 PKHD-TYPE HYDROXYLASE YBIX
1 178 FunFam G3DSA:2.60.120.620:FF:000006 PKHD-type hydroxylase YbiX
78 178 ProSiteProfiles PS51471 Fe(2+) 2-oxoglutarate dioxygenase domain profile.
78 178 InterPro IPR005123 Oxoglutarate/iron-dependent dioxygenase
3 174 SUPERFAMILY SSF51197 Clavaminate synthase-like
180 226 FunFam G3DSA:4.10.860.20:FF:000001 PKHD-type hydroxylase YbiX
180 226 Gene3D G3DSA:4.10.860.20 Rabenosyn, Rab binding domain
1 178 Gene3D G3DSA:2.60.120.620 q2cbj1_9rhob like domain
84 176 Pfam PF13640 2OG-Fe(II) oxygenase superfamily
84 176 InterPro IPR044862 Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain
183 225 Pfam PF18331 PKHD-type hydroxylase C-terminal domain
183 225 InterPro IPR041097 PKHD-type hydroxylase C-terminal domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 9GHL
X-ray 2.00 Å A,B,C,D,E,F
99.6% 2-226
PDBe RCSB PDBj File
Viewing
AlphaFold PA4515
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.619
1 0.383

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 16.61 0.782