Protein profile

PA4528

type 4 prepilin peptidase PilD

Genome: NC_002516.2

Gene: PA4528 pilD xcpA Structure source: AlphaFold UniProt P22610
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Amino acids 290
Annotations 10
Features 36
PDB binders 0
Druggability 0.829

Overview

Basic information about this protein and its source genome.

Accession
PA4528
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4528 pilD xcpA
Status
annotated
Amino acids
290
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0004190 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which a water molecule bound by the side chains of aspartic residues at the active center acts as a nucleophile. GO:0046872 Binding to a metal ion. GO:0008170 Catalysis of the transfer of a methyl group to the nitrogen atom of an acceptor molecule. GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.829
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

10 GO

Gene Ontology (GO)

10
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0004190 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which a water molecule bound by the side chains of aspartic residues at the active center acts as a nucleophile.
  • GO:0046872 Binding to a metal ion.
  • GO:0008170 Catalysis of the transfer of a methyl group to the nitrogen atom of an acceptor molecule.
  • GO:0032259 The process in which a methyl group is covalently attached to a molecule.
  • GO:0009297 The assembly from its constituent parts of a pilus, a short filamentous structure of bacterial cell, flagella-like in structure and generally present in many copies. Pili are variously involved in transfer of nucleic acids, adherence to surfaces, and formation of pellicles. Is required for bacterial conjugation, or can play a role in adherence to surfaces (when it is called a fimbrium), and in the formation of pellicles.
  • GO:0006465 OBSOLETE. The proteolytic removal of a signal peptide from a protein during or after transport to a specific location in the cell.
  • GO:0043683 The assembly from its constituent parts of a type IV pilus.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Sequence Features

Domain/signature hits from InterPro and related databases.

36 records
Show feature table
Start End DB Term Name
155 176 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
216 247 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
149 154 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
128 286 FunFam G3DSA:1.20.120.1220:FF:000001 Type 4 prepilin-like proteins leader peptide-processing enzyme
111 286 Gene3D G3DSA:1.20.120.1220 -
20 126 Pfam PF06750 Bacterial Peptidase A24 N-terminal domain
20 126 InterPro IPR010627 Peptidase A24A, N-terminal
125 129 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
10 32 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
259 276 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
225 240 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
225 240 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
202 212 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
202 212 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
145 162 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
145 162 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
213 224 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
213 224 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
34 107 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
205 215 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
130 148 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
177 181 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
137 245 Pfam PF01478 Type IV leader peptidase family
137 245 InterPro IPR000045 Prepilin type IV endopeptidase, peptidase domain
224 246 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
182 204 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
108 124 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
13 286 PANTHER PTHR30487 TYPE 4 PREPILIN-LIKE PROTEINS LEADER PEPTIDE-PROCESSING ENZYME
158 175 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
259 276 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 33 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
126 148 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
182 204 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
277 290 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
248 258 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4528
AlphaFold — — full sequence — Viewing
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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.829
12 0.372
1 0.239