Protein profile

PA4550

type 4 fimbrial biogenesis protein FimU

Genome: NC_002516.2

Gene: GUL26_14535 DT376_05005 PA4550 ALP65_00673 PAERUG_P19_London_7_VIM_2_05_10_04545 CAZ10_23800 fimU IPC1295_27395 Structure source: Experimental + AlphaFold UniProt G3XCZ0 UniProt Q59652
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Amino acids 168
Annotations 7
Features 15
PDB binders 0
Druggability 0.791

Overview

Basic information about this protein and its source genome.

Accession
PA4550
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
GUL26_14535 DT376_05005 PA4550 ALP65_00673 PAERUG_P19_London_7_VIM_2_05_10_04545 CAZ10_23800 fimU IPC1295_27395
Status
annotated
Amino acids
168
Structure source
Experimental + AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0044096 A short filamentous structure on the surface of a bacterial cell distinguished from other pili by post-translational N-methylation of the pilin monomers. GO:0009297 The assembly from its constituent parts of a pilus, a short filamentous structure of bacterial cell, flagella-like in structure and generally present in many copies. Pili are variously involved in transfer of nucleic acids, adherence to surfaces, and formation of pellicles. Is required for bacterial conjugation, or can play a role in adherence to surfaces (when it is called a fimbrium), and in the formation of pellicles. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways. GO:0043683 The assembly from its constituent parts of a type IV pilus.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.791
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0044096 A short filamentous structure on the surface of a bacterial cell distinguished from other pili by post-translational N-methylation of the pilin monomers.
  • GO:0009297 The assembly from its constituent parts of a pilus, a short filamentous structure of bacterial cell, flagella-like in structure and generally present in many copies. Pili are variously involved in transfer of nucleic acids, adherence to surfaces, and formation of pellicles. Is required for bacterial conjugation, or can play a role in adherence to surfaces (when it is called a fimbrium), and in the formation of pellicles.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.
  • GO:0043683 The assembly from its constituent parts of a type IV pilus.
  • GO:0043107 Any process involved in the controlled movement of a bacterial cell which is dependent on the presence of type IV pili. Includes social gliding motility and twitching motility.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
33 168 Gene3D G3DSA:3.55.40.10 minor pseudopilin epsh domain
34 168 FunFam G3DSA:3.55.40.10:FF:000001 Type 4 fimbrial biogenesis protein FimU
8 28 ProSitePatterns PS00409 Prokaryotic N-terminal methylation site.
8 28 InterPro IPR012902 Prokaryotic N-terminal methylation site
12 30 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
45 153 Pfam PF12019 Type II transport protein GspH
45 153 InterPro IPR022346 General secretion pathway, GspH
4 29 Pfam PF07963 Prokaryotic N-terminal methylation motif
4 29 InterPro IPR012902 Prokaryotic N-terminal methylation site
39 59 Coils Coil Coil
10 140 SUPERFAMILY SSF54523 Pili subunits
10 140 InterPro IPR045584 Pilin-like
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
31 168 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

2 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 4IPU
X-ray 1.35 Å A,B,C,D
78.6% 37-168
PDBe RCSB PDBj File
Viewing
PDB 4IPV
X-ray 1.40 Å A,B
78.6% 37-168
PDBe RCSB PDBj File
Loaded
AlphaFold PA4550
AlphaFold — — full sequence — Loaded
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