Protein profile

PA4562

hypothetical protein

Genome: NC_002516.2

Gene: PA4562 murJ Structure source: AlphaFold UniProt Q9HVM2
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Amino acids 512
Annotations 7
Features 69
PDB binders 2
Druggability 0.947

Overview

Basic information about this protein and its source genome.

Accession
PA4562
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4562 murJ
Status
annotated
Amino acids
512
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015648 Enables the directed movement of lipid-linked peptidoglycans into, out of or within a cell, or between cells. GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. GO:0034204 The translocation, or flipping, of lipid molecules from one monolayer of a membrane bilayer to the opposite monolayer. GO:0015836 The directed movement of lipid-linked peptidoglycans into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0009252 The chemical reactions and pathways resulting in the formation of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.947
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015648 Enables the directed movement of lipid-linked peptidoglycans into, out of or within a cell, or between cells.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
  • GO:0034204 The translocation, or flipping, of lipid molecules from one monolayer of a membrane bilayer to the opposite monolayer.
  • GO:0015836 The directed movement of lipid-linked peptidoglycans into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0009252 The chemical reactions and pathways resulting in the formation of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.
  • GO:0008360 Any process that modulates the surface configuration of a cell.

Sequence Features

Domain/signature hits from InterPro and related databases.

69 records
Show feature table
Start End DB Term Name
274 291 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
132 154 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
134 154 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
3 467 Hamap MF_02078 Probable lipid II flippase MurJ [murJ].
3 467 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
90 112 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
161 182 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 506 PANTHER PTHR47019 LIPID II FLIPPASE MURJ
188 208 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
183 187 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
186 203 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
274 292 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
410 427 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
448 466 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
209 228 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
478 499 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
255 273 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
312 334 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
408 427 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
11 431 CDD cd13123 MATE_MurJ_like
11 431 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
335 353 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
481 503 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
357 376 PRINTS PR01806 Virulence factor MviN signature
357 376 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
409 426 PRINTS PR01806 Virulence factor MviN signature
409 426 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
157 178 PRINTS PR01806 Virulence factor MviN signature
157 178 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
52 68 PRINTS PR01806 Virulence factor MviN signature
52 68 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
133 157 PRINTS PR01806 Virulence factor MviN signature
133 157 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
259 285 PRINTS PR01806 Virulence factor MviN signature
259 285 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
33 52 PRINTS PR01806 Virulence factor MviN signature
33 52 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
467 477 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 512 PIRSF PIRSF002869 MviN
1 512 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
115 133 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
83 114 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
293 312 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
22 30 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
354 371 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
2 502 NCBIfam TIGR01695 murein biosynthesis integral membrane protein MurJ
2 502 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
6 16 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 21 Phobius SIGNAL_PEPTIDE Signal peptide region
52 82 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
383 405 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
500 512 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
383 402 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
313 334 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
159 181 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
354 376 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
26 45 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 5 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
17 21 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
403 407 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
237 259 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
31 51 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
444 466 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
155 160 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
28 476 Pfam PF03023 Lipid II flippase MurJ
28 476 InterPro IPR004268 Peptidoglycan biosynthesis protein MurJ
428 447 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
229 254 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
372 382 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4562
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.947
5 0.296
1 0.248

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
OLB
5T77
B7IE18 356.5 Da LogP 4.92 TPSA 66.8 ✓ Ro5 ✓ Clean CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](CO)O
OLC
5T77
B7IE18 356.5 Da LogP 4.92 TPSA 66.8 ✓ Ro5 ✓ Clean CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](CO)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.