Protein target profile

PA4678

peptide n-acetyltransferase RimI

Genome: NC_002516.2

Gene: PA4678 rimI 3D evidence: AlphaFold DB model UniProt Q9HVB7
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Length 150
Pocket druggability 0.716
EC / GO 1 / 5
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

4 signals

Strengths

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Strong predicted binding pocket FPocket selected-pocket score 0.72. This is a computational pocket signal, not binding validation. Structure available AlphaFold DB model

Risks / watch

No major risk signal surfaced in the loaded evidence.

Missing evidence

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
How to read this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal TPW pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Overview

Basic information about this protein and its source genome.

Accession
PA4678
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4678 rimI
Status
annotated
Amino acids
150
3D evidence
AlphaFold DB model
EC
2.3.1.266
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0008999 Catalysis of the reaction: acetyl-CoA + N-terminal L-alanyl-[protein] = CoA + H+ + N-terminal N(alpha)-acetyl-L-alanyl-[protein]. GO:0016747 Catalysis of the transfer of an acyl group, other than amino-acyl, from one compound (donor) to another (acceptor). GO:0008080 Catalysis of the transfer of an acetyl group to a nitrogen atom on the acceptor molecule. GO:0006474 The acetylation of the N-terminal amino acid of proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
Hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected pocket evidence

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.716
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MSDAISFRPMTEADLDAVLKIEYAAFSHPWTRGIFSEGLKNYHCWLMFEGSQQVGHGVIQIILDEAHLLNITVKPESQGRGLGLRLLEHLMREAAGHQARECFLEVRASNQAAYRLYERYGFNEIGRRRDYYPAVGGREDALVMACTLFD

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0008999 Catalysis of the reaction: acetyl-CoA + N-terminal L-alanyl-[protein] = CoA + H+ + N-terminal N(alpha)-acetyl-L-alanyl-[protein].
  • GO:0016747 Catalysis of the transfer of an acyl group, other than amino-acyl, from one compound (donor) to another (acceptor).
  • GO:0008080 Catalysis of the transfer of an acetyl group to a nitrogen atom on the acceptor molecule.
  • GO:0006474 The acetylation of the N-terminal amino acid of proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

14 records
Show feature table
Start End DB Term Name
6 149 Gene3D G3DSA:3.40.630.30 -
7 148 Hamap MF_02210 [Ribosomal protein S18]-alanine N-acetyltransferase [rimI].
7 148 InterPro IPR043690 N-acetyltransferase RimI
44 105 CDD cd04301 NAT_SF
5 149 ProSiteProfiles PS51186 Gcn5-related N-acetyltransferase (GNAT) domain profile.
5 149 InterPro IPR000182 GNAT domain
5 148 SUPERFAMILY SSF55729 Acyl-CoA N-acyltransferases (Nat)
5 148 InterPro IPR016181 Acyl-CoA N-acyltransferase
8 145 PANTHER PTHR43420 ACETYLTRANSFERASE
14 144 NCBIfam TIGR01575 ribosomal protein S18-alanine N-acetyltransferase
14 144 InterPro IPR006464 N-acetyltransferase RimI/Ard1
29 122 Pfam PF00583 Acetyltransferase (GNAT) family
29 122 InterPro IPR000182 GNAT domain
4 150 FunFam G3DSA:3.40.630.30:FF:000018 [Ribosomal protein S18]-alanine N-acetyltransferase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

3D visualization script Full viewer

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Structural evidence

0 + 1

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB PA4678
AlphaFold DB — — full sequence — Viewing
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Site 1 FPocket #1
0.716
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