Protein profile

PA4745

transcription elongation factor NusA

Genome: NC_002516.2

Gene: PA4745 nusA Structure source: AlphaFold UniProt Q9HV54
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Amino acids 493
Annotations 8
Features 53
PDB binders 0
Druggability 0.887

Overview

Basic information about this protein and its source genome.

Accession
PA4745
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4745 nusA
Status
annotated
Amino acids
493
Structure source
AlphaFold
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. GO:0000166 Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. GO:0003723 Binding to an RNA molecule or a portion thereof. GO:0006353 The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template. GO:0031564 A positive regulation of gene expression mechanism that allows RNA polymerase to continue transcription beyond a termination site, thus allowing expression of downstream genes under specific conditions.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.887
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

8 GO

Gene Ontology (GO)

8
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
  • GO:0000166 Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
  • GO:0003723 Binding to an RNA molecule or a portion thereof.
  • GO:0006353 The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template.
  • GO:0031564 A positive regulation of gene expression mechanism that allows RNA polymerase to continue transcription beyond a termination site, thus allowing expression of downstream genes under specific conditions.
  • GO:0003676 Binding to a nucleic acid.
  • GO:0031554 Any process that modulates the frequency, rate, extent, or location of DNA-templated transcription termination, the process in which transcription is completed; the formation of phosphodiester bonds ceases, the RNA-DNA hybrid dissociates, and RNA polymerase releases the DNA.

Sequence Features

Domain/signature hits from InterPro and related databases.

53 records
Show feature table
Start End DB Term Name
280 348 Gene3D G3DSA:3.30.300.20 -
280 348 InterPro IPR015946 K homology domain-like, alpha/beta
354 423 FunFam G3DSA:1.10.150.20:FF:000018 Transcription termination/antitermination protein NusA
1 128 SUPERFAMILY SSF69705 Transcription factor NusA, N-terminal domain
1 128 InterPro IPR036555 NusA, N-terminal domain superfamily
1 389 PANTHER PTHR22648 TRANSCRIPTION TERMINATION FACTOR NUSA
1 389 InterPro IPR030842 Transcription termination/antitermination protein NusA, bacterial
280 348 FunFam G3DSA:3.30.300.20:FF:000005 Transcription termination/antitermination protein NusA
367 415 NCBIfam TIGR01954 transcription termination factor NusA, C-terminal duplication
367 415 InterPro IPR010214 Transcription termination factor NusA, C-terminal duplication
442 491 NCBIfam TIGR01954 transcription termination factor NusA, C-terminal duplication
442 491 InterPro IPR010214 Transcription termination factor NusA, C-terminal duplication
1 131 Gene3D G3DSA:3.30.1480.10 -
1 131 InterPro IPR036555 NusA, N-terminal domain superfamily
434 488 Pfam PF14520 Helix-hairpin-helix domain
433 491 FunFam G3DSA:1.10.150.20:FF:000015 Transcription termination/antitermination protein NusA
135 197 Pfam PF00575 S1 RNA binding domain
135 197 InterPro IPR003029 S1 domain
357 418 SUPERFAMILY SSF47794 Rad51 N-terminal domain-like
357 418 InterPro IPR010995 DNA repair Rad51/transcription factor NusA, alpha-helical
5 344 NCBIfam TIGR01953 transcription termination factor NusA
5 344 InterPro IPR010213 Transcription termination factor NusA
201 279 FunFam G3DSA:3.30.300.20:FF:000002 Transcription termination/antitermination protein NusA
304 340 ProSiteProfiles PS50084 Type-1 KH domain profile.
356 423 Gene3D G3DSA:1.10.150.20 -
201 279 Gene3D G3DSA:3.30.300.20 -
201 279 InterPro IPR015946 K homology domain-like, alpha/beta
135 202 SMART SM00316 S1_6
135 202 InterPro IPR022967 RNA-binding domain, S1
134 201 CDD cd04455 S1_NusA
232 299 Pfam PF13184 NusA-like KH domain
232 299 InterPro IPR025249 KH domain, NusA-like
1 131 FunFam G3DSA:3.30.1480.10:FF:000001 Transcription termination/antitermination protein NusA
134 201 SUPERFAMILY SSF50249 Nucleic acid-binding proteins
134 201 InterPro IPR012340 Nucleic acid-binding, OB-fold
281 341 CDD cd22529 KH-II_NusA_rpt2
432 492 SUPERFAMILY SSF47794 Rad51 N-terminal domain-like
432 492 InterPro IPR010995 DNA repair Rad51/transcription factor NusA, alpha-helical
137 202 ProSiteProfiles PS50126 S1 domain profile.
137 202 InterPro IPR003029 S1 domain
202 280 SUPERFAMILY SSF54814 Prokaryotic type KH domain (KH-domain type II)
202 280 InterPro IPR009019 K homology domain superfamily, prokaryotic type
2 412 Hamap MF_00945_B Transcription termination/antitermination protein NusA [nusA].
2 412 InterPro IPR030842 Transcription termination/antitermination protein NusA, bacterial
433 491 Gene3D G3DSA:1.10.150.20 -
131 200 FunFam G3DSA:2.40.50.140:FF:000058 Transcription termination/antitermination protein NusA
281 344 SUPERFAMILY SSF54814 Prokaryotic type KH domain (KH-domain type II)
281 344 InterPro IPR009019 K homology domain superfamily, prokaryotic type
132 200 Gene3D G3DSA:2.40.50.140 -
132 200 InterPro IPR012340 Nucleic acid-binding, OB-fold
204 278 CDD cd02134 KH-II_NusA_rpt1
4 125 Pfam PF08529 NusA N-terminal domain
4 125 InterPro IPR013735 Transcription factor NusA, N-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4745
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.672
2 0.341