Protein profile

PA4748

triosephosphate isomerase

Genome: NC_002516.2

Gene: PA4748 tpiA Structure source: AlphaFold UniProt Q9HV51
Export view
Amino acids 251
Annotations 7
Features 19
PDB binders 3
Druggability 0.452

Overview

Basic information about this protein and its source genome.

Accession
PA4748
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4748 tpiA
Status
annotated
Amino acids
251
Structure source
AlphaFold
EC
5.3.1.1
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0004807 Catalysis of the reaction: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate. GO:0006094 The formation of glucose from noncarbohydrate precursors, such as pyruvate, amino acids and glycerol. GO:0046166 The chemical reactions and pathways resulting in the formation of glyceraldehyde-3-phosphate, an important intermediate in glycolysis. GO:0019563 The chemical reactions and pathways resulting in the breakdown of glycerol, 1,2,3-propanetriol, a sweet, hygroscopic, viscous liquid, widely distributed in nature as a constituent of many lipids. GO:0006096 The chemical reactions and pathways resulting in the breakdown of a carbohydrate into pyruvate, with the concomitant production of a small amount of ATP and the reduction of NAD(P) to NAD(P)H. Glycolysis begins with the metabolism of a carbohydrate to generate products that can enter the pathway and ends with the production of pyruvate. Pyruvate may be converted to acetyl-coenzyme A, ethanol, lactate, or other small molecules.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
49.123
Human E-value
6.549999999999999e-30
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.452
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0004807 Catalysis of the reaction: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate.
  • GO:0006094 The formation of glucose from noncarbohydrate precursors, such as pyruvate, amino acids and glycerol.
  • GO:0046166 The chemical reactions and pathways resulting in the formation of glyceraldehyde-3-phosphate, an important intermediate in glycolysis.
  • GO:0019563 The chemical reactions and pathways resulting in the breakdown of glycerol, 1,2,3-propanetriol, a sweet, hygroscopic, viscous liquid, widely distributed in nature as a constituent of many lipids.
  • GO:0006096 The chemical reactions and pathways resulting in the breakdown of a carbohydrate into pyruvate, with the concomitant production of a small amount of ATP and the reduction of NAD(P) to NAD(P)H. Glycolysis begins with the metabolism of a carbohydrate to generate products that can enter the pathway and ends with the production of pyruvate. Pyruvate may be converted to acetyl-coenzyme A, ethanol, lactate, or other small molecules.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
1 250 FunFam G3DSA:3.20.20.70:FF:000016 Triosephosphate isomerase
1 248 SUPERFAMILY SSF51351 Triosephosphate isomerase (TIM)
1 248 InterPro IPR035990 Triosephosphate isomerase superfamily
2 248 Hamap MF_00147_B Triosephosphate isomerase [tpiA].
2 248 InterPro IPR022896 Triosephosphate isomerase, bacterial/eukaryotic
4 247 CDD cd00311 TIM
4 247 InterPro IPR000652 Triosephosphate isomerase
3 248 ProSiteProfiles PS51440 Triosephosphate isomerase (TIM) family profile.
3 248 InterPro IPR000652 Triosephosphate isomerase
1 250 Gene3D G3DSA:3.20.20.70 Aldolase class I
1 250 InterPro IPR013785 Aldolase-type TIM barrel
5 240 NCBIfam TIGR00419 triose-phosphate isomerase
5 240 InterPro IPR000652 Triosephosphate isomerase
165 175 ProSitePatterns PS00171 Triosephosphate isomerase active site.
165 175 InterPro IPR020861 Triosephosphate isomerase, active site
2 247 PANTHER PTHR21139 TRIOSEPHOSPHATE ISOMERASE
2 247 InterPro IPR000652 Triosephosphate isomerase
4 248 Pfam PF00121 Triosephosphate isomerase
4 248 InterPro IPR000652 Triosephosphate isomerase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4748
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.452
4 0.289

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

29 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
13P
5UJW
Q5NII7 170.1 Da LogP -1.34 TPSA 104.1 ✓ Ro5 ✓ Clean C(C(=O)COP(=O)(O)O)O
PEP
4OWG
P00939 168.0 Da LogP -0.31 TPSA 104.1 ✓ Ro5 ✓ Clean C=C(C(=O)O)OP(=O)(O)O
PGA
6OOI
P48501 156.0 Da LogP -0.82 TPSA 104.1 ✓ Ro5 ✓ Clean C(C(=O)O)OP(=O)(O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.