Protein profile

PA4852

hypothetical protein

Genome: NC_002516.2

Gene: dusB PA4852 Structure source: AlphaFold UniProt Q9HUW1
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Amino acids 332
Annotations 8
Features 18
PDB binders 0
Druggability 0.541

Overview

Basic information about this protein and its source genome.

Accession
PA4852
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
dusB PA4852
Status
annotated
Amino acids
332
Structure source
AlphaFold
EC
1.3.1.-
GO
GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2. GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes. GO:0000049 Binding to a transfer RNA. GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H. GO:0016491 Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced. GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
38.095
Human E-value
2.78e-13
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.541
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
  • GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
  • GO:0000049 Binding to a transfer RNA.
  • GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H.
  • GO:0016491 Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
  • GO:0002943 The process whereby a uridine in a transfer RNA is converted to dihydrouridine.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
10 250 Gene3D G3DSA:3.20.20.70 Aldolase class I
10 250 InterPro IPR013785 Aldolase-type TIM barrel
6 321 NCBIfam TIGR00737 tRNA dihydrouridine synthase DusB
6 321 InterPro IPR004652 tRNA-dihydrouridine synthase DusB-like
12 306 PANTHER PTHR45846 TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE
14 244 CDD cd02801 DUS_like_FMN
14 244 InterPro IPR035587 DUS-like, FMN-binding domain
16 320 Pfam PF01207 Dihydrouridine synthase (Dus)
16 320 InterPro IPR035587 DUS-like, FMN-binding domain
4 324 Hamap MF_02042 tRNA-dihydrouridine synthase B [dusB].
4 324 InterPro IPR032887 tRNA-dihydrouridine synthase B
97 115 ProSitePatterns PS01136 Uncharacterized protein family UPF0034 signature.
97 115 InterPro IPR018517 tRNA-dihydrouridine synthase, conserved site
3 330 PIRSF PIRSF006621 Dus
3 330 InterPro IPR001269 tRNA-dihydrouridine synthase
13 322 SUPERFAMILY SSF51395 FMN-linked oxidoreductases
256 328 Gene3D G3DSA:1.10.1200.80 Putative flavin oxidoreducatase; domain 2
256 328 InterPro IPR024036 tRNA-dihydrouridine synthase, putative, C-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4852
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.485
3 0.32