Protein profile

PA4886

two-component sensor

Genome: NC_002516.2

Gene: PA4886 Structure source: AlphaFold UniProt Q9HUS7
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Amino acids 463
Annotations 10
Features 44
PDB binders 3
Druggability 0.858

Overview

Basic information about this protein and its source genome.

Accession
PA4886
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA4886
Status
annotated
Amino acids
463
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.858
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

9
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0004673 Catalysis of the reaction: ATP + protein L-histidine = ADP + protein phospho-L-histidine.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Sequence Features

Domain/signature hits from InterPro and related databases.

44 records
Show feature table
Start End DB Term Name
311 463 Gene3D G3DSA:3.30.565.10 -
311 463 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
4 460 NCBIfam TIGR01386 heavy metal sensor histidine kinase
4 460 InterPro IPR006290 Heavy metal sensor kinase
250 463 ProSiteProfiles PS50109 Histidine kinase domain profile.
250 463 InterPro IPR005467 Histidine kinase domain
191 239 SUPERFAMILY SSF158472 HAMP domain-like
423 441 PRINTS PR00344 Bacterial sensor protein C-terminal signature
423 441 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
407 417 PRINTS PR00344 Bacterial sensor protein C-terminal signature
407 417 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
169 188 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
159 244 Gene3D G3DSA:6.10.340.10 -
314 460 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
314 460 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
243 309 SMART SM00388 HisKA_10
243 309 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
10 32 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
2 462 PANTHER PTHR45436 SENSOR HISTIDINE KINASE YKOH
226 307 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
226 307 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
276 296 Coils Coil Coil
169 188 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
32 168 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
189 463 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
242 305 CDD cd00082 HisKA
242 305 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
192 237 CDD cd06225 HAMP
244 307 Pfam PF00512 His Kinase A (phospho-acceptor) domain
244 307 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
245 308 Gene3D G3DSA:1.10.287.130 -
353 448 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
353 448 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
357 457 CDD cd00075 HATPase
240 310 FunFam G3DSA:1.10.287.130:FF:000001 Two-component sensor histidine kinase
189 242 ProSiteProfiles PS50885 HAMP domain profile.
189 242 InterPro IPR003660 HAMP domain
187 238 Pfam PF00672 HAMP domain
187 238 InterPro IPR003660 HAMP domain
352 461 SMART SM00387 HKATPase_4
352 461 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
189 242 SMART SM00304 HAMP_11

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA4886
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
12 0.354
2 0.334
11 0.261

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO
RDC
3CGY
P0DM80 364.8 Da LogP 2.69 TPSA 96.4 ✓ Ro5 ✓ Clean C[C@@H]1C[C@@H]2[C@H](O2)\C=C/C=C/C(=O)Cc3c(c(c…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.