Protein profile

PA4945

tRNA delta(2)-isopentenylpyrophosphate transferase

Genome: NC_002516.2

Gene: miaA PA4945 Structure source: Experimental + AlphaFold UniProt Q9HUL9
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Amino acids 323
Annotations 5
Features 13
PDB binders 5
Druggability 0.373

Overview

Basic information about this protein and its source genome.

Accession
PA4945
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
miaA PA4945
Status
annotated
Amino acids
323
Structure source
Experimental + AlphaFold
EC
2.5.1.75
GO
GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0052381 Catalysis of the reaction: adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate. GO:0006400 The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically. GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
37.383
Human E-value
5.24e-16
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.373
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0052381 Catalysis of the reaction: adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate.
  • GO:0006400 The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.

Sequence Features

Domain/signature hits from InterPro and related databases.

13 records
Show feature table
Start End DB Term Name
6 296 PANTHER PTHR11088 TRNA DIMETHYLALLYLTRANSFERASE
6 296 InterPro IPR039657 Dimethylallyltransferase
7 303 NCBIfam TIGR00174 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
7 303 InterPro IPR018022 IPP transferase
6 318 Gene3D G3DSA:3.40.50.300 -
6 318 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
115 185 FunFam G3DSA:1.10.20.140:FF:000001 tRNA dimethylallyltransferase
7 314 Hamap MF_00185 tRNA dimethylallyltransferase [miaA].
7 314 InterPro IPR018022 IPP transferase
115 184 Gene3D G3DSA:1.10.20.140 -
41 294 Pfam PF01715 IPP transferase
8 152 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
8 152 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

3 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 3CRM
X-ray 1.90 Å A
100.0% 1-323
PDBe RCSB PDBj File
Viewing
PDB 3CRR
X-ray 1.90 Å A
100.0% 1-323
PDBe RCSB PDBj File
Loaded
PDB 3CRQ
X-ray 2.20 Å A
100.0% 1-323
PDBe RCSB PDBj File
Loaded
AlphaFold PA4945
AlphaFold — — full sequence — Loaded
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Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

55 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

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Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
DPO
3CRQ 2.20 Å Open crystal
173.9 Da LogP -3.34 TPSA 135.6 ✓ Ro5 ✓ Clean [O-]P(=O)([O-])OP(=O)([O-])[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.