Protein profile

PA5014

bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase

Genome: NC_002516.2

Gene: glnE PA5014 Structure source: AlphaFold UniProt Q9HUF4
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Amino acids 982
Annotations 7
Features 35
PDB binders 0
Druggability 0.478

Overview

Basic information about this protein and its source genome.

Accession
PA5014
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
glnE PA5014
Status
annotated
Amino acids
982
Structure source
AlphaFold
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0008882 Catalysis of the reaction: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]. GO:0047388 Catalysis of the reaction: [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000287 Binding to a magnesium (Mg) ion. GO:0000820 OBSOLETE. Any process that modulates the frequency, rate or extent of the chemical reactions and pathways involving amino acids of the glutamine family, comprising arginine, glutamate, glutamine and proline.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.478
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0008882 Catalysis of the reaction: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)].
  • GO:0047388 Catalysis of the reaction: [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000287 Binding to a magnesium (Mg) ion.
  • GO:0000820 OBSOLETE. Any process that modulates the frequency, rate or extent of the chemical reactions and pathways involving amino acids of the glutamine family, comprising arginine, glutamate, glutamine and proline.
  • GO:0016779 Catalysis of the transfer of a nucleotidyl group from one compound (donor) to another (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

35 records
Show feature table
Start End DB Term Name
45 982 Hamap MF_00802 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme [glnE].
45 982 InterPro IPR023057 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
837 980 Gene3D G3DSA:1.20.120.330 Nucleotidyltransferases domain 2
57 300 Gene3D G3DSA:3.30.460.10 Beta Polymerase, domain 2
57 300 InterPro IPR043519 Nucleotidyltransferase superfamily
586 836 Gene3D G3DSA:3.30.460.10 Beta Polymerase, domain 2
586 836 InterPro IPR043519 Nucleotidyltransferase superfamily
586 836 FunFam G3DSA:3.30.460.10:FF:000009 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
305 459 FunFam G3DSA:1.20.120.330:FF:000005 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
307 456 SUPERFAMILY SSF81593 Nucleotidyltransferase substrate binding subunit/domain
57 303 FunFam G3DSA:3.30.460.10:FF:000009 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
29 306 SUPERFAMILY SSF81301 Nucleotidyltransferase
29 306 InterPro IPR043519 Nucleotidyltransferase superfamily
1 19 Phobius SIGNAL_PEPTIDE Signal peptide region
317 456 Pfam PF08335 GlnD PII-uridylyltransferase
317 456 InterPro IPR013546 PII-uridylyltransferase/Glutamine-synthetase adenylyltransferase
852 955 Pfam PF08335 GlnD PII-uridylyltransferase
852 955 InterPro IPR013546 PII-uridylyltransferase/Glutamine-synthetase adenylyltransferase
840 979 SUPERFAMILY SSF81593 Nucleotidyltransferase substrate binding subunit/domain
305 459 Gene3D G3DSA:1.20.120.330 Nucleotidyltransferases domain 2
4 979 PANTHER PTHR30621 GLUTAMINE SYNTHETASE ADENYLYLTRANSFERASE
4 979 InterPro IPR023057 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
643 835 CDD cd05401 NT_GlnE_GlnD_like
3 14 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
474 585 Gene3D G3DSA:1.20.120.1510 -
117 302 CDD cd05401 NT_GlnE_GlnD_like
48 296 Pfam PF03710 Glutamate-ammonia ligase adenylyltransferase
48 296 InterPro IPR005190 Glutamate-ammonia ligase adenylyltransferase, repeated domain
577 830 Pfam PF03710 Glutamate-ammonia ligase adenylyltransferase
577 830 InterPro IPR005190 Glutamate-ammonia ligase adenylyltransferase, repeated domain
549 840 SUPERFAMILY SSF81301 Nucleotidyltransferase
549 840 InterPro IPR043519 Nucleotidyltransferase superfamily
20 982 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
15 19 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 2 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5014
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.478
5 0.229