Protein profile

PA5044

type 4 fimbrial biogenesis protein PilM

Genome: NC_002516.2

Gene: pilM PA5044 GUL26_08165 DY940_17240 IPC1295_08195 DT376_01065 PAERUG_P19_London_7_VIM_2_05_10_03104 GNQ48_04060 L4V69_34510 Structure source: Experimental + AlphaFold UniProt G3XD28 UniProt Q51351
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Amino acids 354
Annotations 8
Features 18
PDB binders 1
Druggability 0.754

Overview

Basic information about this protein and its source genome.

Accession
PA5044
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
pilM PA5044 GUL26_08165 DY940_17240 IPC1295_08195 DT376_01065 PAERUG_P19_London_7_VIM_2_05_10_03104 GNQ48_04060 L4V69_34510
Status
annotated
Amino acids
354
Structure source
Experimental + AlphaFold
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0044096 A short filamentous structure on the surface of a bacterial cell distinguished from other pili by post-translational N-methylation of the pilin monomers. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0046872 Binding to a metal ion. GO:0051301 The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells. GO:0043683 The assembly from its constituent parts of a type IV pilus.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.754
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVVAVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALALAGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSVEEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANPFADMALNGKVNAGALASDAPALMIACGLALRSFD

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

8 GO

Gene Ontology (GO)

8
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0044096 A short filamentous structure on the surface of a bacterial cell distinguished from other pili by post-translational N-methylation of the pilin monomers.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0046872 Binding to a metal ion.
  • GO:0051301 The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
  • GO:0043683 The assembly from its constituent parts of a type IV pilus.
  • GO:0043107 Any process involved in the controlled movement of a bacterial cell which is dependent on the presence of type IV pili. Includes social gliding motility and twitching motility.
  • GO:0005515 Binding to a protein.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
14 353 Pfam PF11104 Type IV pilus assembly protein PilM;
14 353 InterPro IPR005883 Type IV pilus inner membrane component PilM
1 354 PIRSF PIRSF019169 Tfp_PilM
1 354 InterPro IPR005883 Type IV pilus inner membrane component PilM
12 181 SMART SM00842 FtsA._2
12 181 InterPro IPR003494 SHS2 domain inserted in FtsA
193 352 SUPERFAMILY SSF53067 Actin-like ATPase domain
193 352 InterPro IPR043129 ATPase, nucleotide binding domain
13 351 Gene3D G3DSA:3.30.420.40 -
88 147 FunFam G3DSA:3.30.1490.300:FF:000001 Type 4 fimbrial biogenesis protein PilM
189 317 FunFam G3DSA:3.30.420.40:FF:000149 Type IV pilus assembly protein PilM
12 177 SUPERFAMILY SSF53067 Actin-like ATPase domain
12 177 InterPro IPR043129 ATPase, nucleotide binding domain
8 353 NCBIfam TIGR01175 type IV pilus assembly protein PilM
8 353 InterPro IPR005883 Type IV pilus inner membrane component PilM
189 317 Gene3D G3DSA:3.30.420.40 -
88 147 Gene3D G3DSA:3.30.1490.300 -
8 353 PANTHER PTHR32432 CELL DIVISION PROTEIN FTSA-RELATED

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

4 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 5EOU
X-ray 2.40 Å A,B
100.0% 1-354
PDBe RCSB PDBj File
Viewing
PDB 5EOX
X-ray 2.40 Å A,B
100.0% 1-354
PDBe RCSB PDBj File
Loaded
PDB 5EOY
X-ray 2.50 Å A,B
100.0% 1-354
PDBe RCSB PDBj File
Loaded
PDB 5EQ6
X-ray 3.50 Å A,B
100.0% 1-354
PDBe RCSB PDBj File
Loaded
AlphaFold PA5044
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 21.42 0.869
2 2.04 0.045

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

51 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Show only:
Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
ANP
5EQ6 3.50 Å Open crystal
506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.