Protein profile

PA5100

urocanate hydratase

Genome: NC_002516.2

Gene: hutU PA5100 Structure source: AlphaFold UniProt Q9HU83
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Amino acids 559
Annotations 6
Features 23
PDB binders 2
Druggability 0.848

Overview

Basic information about this protein and its source genome.

Accession
PA5100
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
hutU PA5100
Status
annotated
Amino acids
559
Structure source
AlphaFold
EC
4.2.1.49
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0016153 Catalysis of the reaction: 4-imidazolone-5-propanoate + H+ = trans-urocanate + H2O. GO:0006548 The chemical reactions and pathways resulting in the breakdown of L-histidine. GO:0019556 OBSOLETE. The chemical reactions and pathways resulting in the breakdown of L-histidine into other compounds, including glutamate and formamide. GO:0019557 OBSOLETE. The chemical reactions and pathways resulting in the breakdown of L-histidine into other compounds, including glutamate and formate.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
33.688
Human E-value
2.1e-96
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.848
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0016153 Catalysis of the reaction: 4-imidazolone-5-propanoate + H+ = trans-urocanate + H2O.
  • GO:0006548 The chemical reactions and pathways resulting in the breakdown of L-histidine.
  • GO:0019556 OBSOLETE. The chemical reactions and pathways resulting in the breakdown of L-histidine into other compounds, including glutamate and formamide.
  • GO:0019557 OBSOLETE. The chemical reactions and pathways resulting in the breakdown of L-histidine into other compounds, including glutamate and formate.

Sequence Features

Domain/signature hits from InterPro and related databases.

23 records
Show feature table
Start End DB Term Name
142 349 Gene3D G3DSA:3.40.50.10730 Urocanase like domains
142 349 InterPro IPR038364 Urocanase, central domain superfamily
13 553 Gene3D G3DSA:3.40.1770.10 Urocanase superfamily
142 349 FunFam G3DSA:3.40.50.10730:FF:000001 Urocanate hydratase
442 457 ProSitePatterns PS01233 Urocanase signature.
442 457 InterPro IPR023636 Urocanase conserved site
9 556 PANTHER PTHR12216 UROCANATE HYDRATASE
9 556 InterPro IPR023637 Urocanase
11 554 NCBIfam TIGR01228 urocanate hydratase
11 554 InterPro IPR023637 Urocanase
212 232 Coils Coil Coil
352 546 Pfam PF17392 Urocanase C-terminal domain
352 546 InterPro IPR035401 Urocanase, C-terminal domain
2 559 PIRSF PIRSF001423 Urocanate_hydrat
2 559 InterPro IPR023637 Urocanase
12 138 Pfam PF17391 Urocanase N-terminal domain
12 138 InterPro IPR035400 Urocanase, N-terminal domain
141 349 Pfam PF01175 Urocanase Rossmann-like domain
141 349 InterPro IPR035085 Urocanase, Rossmann-like domain
8 555 SUPERFAMILY SSF111326 Urocanase
8 555 InterPro IPR036190 Urocanase superfamily
3 556 Hamap MF_00577 Urocanate hydratase [hutU].
3 556 InterPro IPR023637 Urocanase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA5100
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.848
2 0.561
9 0.466

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

37 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
SIN
7JFZ
Q5ZVR1 118.1 Da LogP -0.06 TPSA 74.6 ✓ Ro5 ✓ Clean C(CC(=O)O)C(=O)O
URO
7NED
A0A0Q9KFZ4 138.1 Da LogP 0.51 TPSA 66.0 ✓ Ro5 ✓ Clean c1c(nc[nH]1)C=CC(=O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.