Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA5118
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: thiI PA5118
Status: annotated
Amino acids: 484
Structure source: AlphaFold

EC

2.8.1.4

GO

GO:1990228 A protein complex capable of catalyzing the transfer of sulfur atoms from one compound (donor) to another (acceptor). GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0004810 Catalysis of the reaction: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate. GO:0016783 Catalysis of the transfer of sulfur atoms from one compound (donor) to another (acceptor). GO:0000049 Binding to a transfer RNA. GO:0140741 Catalyzes the reaction: ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): N
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.529

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 12 GO

Enzyme Commission (EC)

1

2.8.1.4

Gene Ontology (GO)

12

GO:1990228 A protein complex capable of catalyzing the transfer of sulfur atoms from one compound (donor) to another (acceptor).
GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0004810 Catalysis of the reaction: a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate.
GO:0016783 Catalysis of the transfer of sulfur atoms from one compound (donor) to another (acceptor).
GO:0000049 Binding to a transfer RNA.
GO:0140741 Catalyzes the reaction: ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.
GO:0009228 The chemical reactions and pathways resulting in the formation of thiamine (vitamin B1), a water soluble vitamin present in fresh vegetables and meats, especially liver.
GO:0009229 The chemical reactions and pathways resulting in the formation of thiamine diphosphate, a derivative of thiamine (vitamin B1) which acts as a coenzyme in a range of processes including the Krebs cycle.
GO:0052837 The chemical reactions and pathways resulting in the formation of a thiazole, a five-membered heterocyclic ring structure containing a sulfur in the 1-position and a nitrogen in the 3-position.
GO:0002937 The processes whereby a uridine residue in a tRNA is converted to 4-thiouridine. Typically 4-thiouridine is found at position 8, in many transfer RNAs.
GO:0034227 The addition a sulfur atom to a nucleotide in a tRNA molecule.
GO:0003723 Binding to an RNA molecule or a portion thereof.

Sequence Features

Domain/signature hits from InterPro and related databases.

31 records

Show feature table

Start	End	DB	Term	Name
1	176	Gene3D	G3DSA:3.30.2130.30	-
1	175	SUPERFAMILY	SSF143437	THUMP domain-like
63	167	ProSiteProfiles	PS51165	THUMP domain profile.
63	167	InterPro	IPR004114	THUMP domain
178	370	Pfam	PF02568	Thiamine biosynthesis protein (ThiI)
178	370	InterPro	IPR020536	Thil, AANH domain
29	166	Pfam	PF02926	THUMP domain
29	166	InterPro	IPR004114	THUMP domain
181	374	SUPERFAMILY	SSF52402	Adenine nucleotide alpha hydrolases-like
182	355	CDD	cd01712	ThiI
182	355	InterPro	IPR020536	Thil, AANH domain
1	399	PANTHER	PTHR43209	TRNA SULFURTRANSFERASE
405	481	ProSiteProfiles	PS50206	Rhodanese domain profile.
405	481	InterPro	IPR001763	Rhodanese-like domain
77	167	SMART	SM00981	THUMP_a_2
77	167	InterPro	IPR004114	THUMP domain
388	483	Gene3D	G3DSA:3.40.250.10	-
388	483	InterPro	IPR036873	Rhodanese-like domain superfamily
397	482	SUPERFAMILY	SSF52821	Rhodanese/Cell cycle control phosphatase
397	482	InterPro	IPR036873	Rhodanese-like domain superfamily
364	384	Coils	Coil	Coil
177	387	Gene3D	G3DSA:3.40.50.620	HUPs
177	387	InterPro	IPR014729	Rossmann-like alpha/beta/alpha sandwich fold
2	172	CDD	cd11716	THUMP_ThiI
386	483	NCBIfam	TIGR04271	thiazole biosynthesis domain
386	483	InterPro	IPR026340	Thiazole biosynthesis domain
1	484	Hamap	MF_00021	tRNA sulfurtransferase [thiI].
1	484	InterPro	IPR003720	tRNA sulfurtransferase ThiI
4	371	NCBIfam	TIGR00342	tRNA 4-thiouridine(8) synthase ThiI
4	371	InterPro	IPR003720	tRNA sulfurtransferase ThiI
177	402	FunFam	G3DSA:3.40.50.620:FF:000029	tRNA sulfurtransferase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA5118	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
2				0.529
1				0.238

PA5118